Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Variable valve apparatus and methods

a valve and variable technology, applied in the field of sample processing devices, can solve the problems of fluid transfer between different features of the device, transfer process, high cost, complexity and associated high cost, and achieve the effect of lowering the surface or interfacial tension of the medium

Inactive Publication Date: 2005-06-16
3M INNOVATIVE PROPERTIES CO
View PDF98 Cites 70 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007] The openings in the valve septum may be formed at locations based on one or more characteristics of the sample material detected within the process chamber. It may be preferred that the process chambers include detection windows that transmit light into and / or out of the process chamber. Detected characteristics of the sample material may include, e.g., the free surface of the sample material (indicative of the volume of sample material in the process chamber). Forming an opening in the valve septum at a selected distance radially outward of the free surface can provide the ability to remove a selected volume of the sample material from the process chamber.
[0010] The openings in the valve septums are preferably formed in the absence of physical contact, e.g., through laser ablation, focused optical heating, etc. As a result, the openings can preferably be formed without piercing the outermost layers of the sample processing device, thus limiting the possibility of leakage of the sample material from the sample processing device.
[0029]“Surfactant” refers to a substance that lowers the surface or interfacial tension of the medium in which it is dissolved.

Problems solved by technology

One persistent issue associated with sample processing devices including process chambers is in the transfer of fluids between different features in the devices.
Such transfer processes suffer from a number of disadvantages including, but not limited to, the potential for errors, complexity and associated high costs, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Variable valve apparatus and methods
  • Variable valve apparatus and methods
  • Variable valve apparatus and methods

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Solid Phase Material: Ammonia Form with TRITON-X 100

[0183] A 3M No. 2271 EMPORE Extraction Chelating Disk was placed in a glass filter holder. The extraction disk was converted into the ammonia form, following the procedure printed on the package insert. The disk placed in a vial and was submerged in a 1% TRITON-X 100 (Sigma-Aldrich, St. Louis, Mo.) solution (0.1 gram (g) of TRITON-X 100 in 10 mL of water), mixing for about 6-8 hours on a Thermolyne Vari-Mix Model M48725 Rocker (Barnstead / Thermolyne, Dubuque, Iowa). The disk was placed in glass filter holder, dried by applying a vacuum for about 20 minutes (min), and then dried overnight at room temperature (approximately 21° C.), taking care not to wash or rinse the disk.

example 2a

Effect of Inhibitor / DNA on PCR: Varying Inhibitor Concentration with Fixed DNA Concentration

[0184] A dilution series of inhibitors were made prior to spiking with clean human genomic DNA in order to study the effect of inhibitor on PCR. To 10 μL of 15 nanograms per microliter (ng / μL) human genomic DNA, 1μL of different Mix I (neat or dilutions thereof) was added (Samples 2—no inhibitor added, 2D—neat, 2E—1:10, 2F—1:30, 2G—1:100, 2H—1:300) and vortexed. Two (2) μL aliquots of each sample were taken for 20 μL PCR. The results are shown in Table 2.

[0185] Mix I: one hundred (100) μL of whole blood was added to 1 μL of neat TRITON-X 100. The solution was incubated at room temperature (approximately 21° C.) for about 5 minutes, vortexing the solution intermittently (for approximately 5 seconds every 20 seconds). The solution was investigated to make sure that it was transparent before proceeding to the next step. The solution was spun in an Eppendorf Model 5415D centrifuge at 400 rcf fo...

example 2b

Effect of Inhibitor / DNA on PCR: Varying DNA Concentration with Fixed Inhibitor Concentration

[0186] To 10 μL of human genomic DNA, 1 μL of 1:3 diluted Mix I (described above) was added. DNA concentrations that were examined were the following: Samples 2J—15 ng / μL, 2K—7.5 ng / μL, 2L—3.75 ng / μL, 2M—1.5 ng / μL. Two (2) μL aliquots of each sample were taken for 20 μL PCR. The results are shown in Table 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
Fractionaaaaaaaaaa
Molar densityaaaaaaaaaa
Login to View More

Abstract

Sample processing devices with variable valve structures and methods of using the same are disclosed. The valve structures allow for removal of selected portions of the sample material located within the process chamber. Removal of the selected portions is achieved by forming an opening in a valve septum at a desired location. The valve septums may be large enough to allow for adjustment of the location of the opening based on the characteristics of the sample material in the process chamber. If the sample processing device is rotated after the opening is formed, the selected portion of the material located closer to the axis of rotation exits the process chamber through the opening. The remainder of the sample material cannot exit through the opening because it is located farther from the axis of rotation than the opening.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] The present application is a Continuation-In-Part of U.S. patent application Ser. No. 10 / 734,717, filed on Dec. 12, 2003, and claims priority to U.S. Provisional Patent Application Ser. No. 60 / 532,523, filed on Dec. 24, 2003, both of which are incorporated herein by reference in their entireties.BACKGROUND [0002] Sample processing devices including process chambers in which various chemical or biological processes are performed play an increasing role in scientific and / or diagnostic investigations. The process chambers provided in such devices are preferably small in volume to reduce the amount of sample material required to perform the processes. [0003] One persistent issue associated with sample processing devices including process chambers is in the transfer of fluids between different features in the devices. Conventional approaches to separate and transfer fluidic contents of process chambers have often required human intervention ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01L3/00C12M1/34C12N15/10C12Q1/68G01N30/00
CPCB01L3/502738B01L2400/0677B01L2400/0409B01L2300/0806
Inventor BEDINGHAM, WILLIAMROBOLE, BARRY W.PARTHASARATHY, RANJANI V.ERICSON, KATYA
Owner 3M INNOVATIVE PROPERTIES CO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com