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Pleuripotent stem cells generated from adipose tissue-derived stromal cells and uses thereof

a technology of stromal cells and pleuripotent stem cells, which is applied in the direction of skeletal/connective tissue cells, nervous system cells, biocide, etc., can solve the problems of high risk and discomfort of patients after bone marrow stromal cells are extracted

Inactive Publication Date: 2006-10-12
ARTECEL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the absence of treatment, these animals died because they failed to replenish their circulating blood cells; however, transplantation of bone marrow cells from syngeneic donor animals rescued the host animal.
However, extraction of bone marrow stromal cells presents a high level of risk and discomfort to the patient.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Hematopoietic Commitment by Adipose Tissue-Derived Stromal Cells

[0065] A. Stromal cells are isolated from human adipose tissue according to the methods described in U.S. patent application Ser. No. 09 / 240,029, Filed Jan. 29, 1999 (the contents of which are incorporated by reference), and using the modifications to the growth medium as described above. Briefly, human preadipocytes were isolated from adipose tissue removed by liposuction surgery according to the procedures previously described by Rodbell and Hauner (Rodbell (1967) and (1974); Hauner, supra). Preadipocytes from the stromal-vascular fraction were resuspended in DMEM (high glucose) media containing 10% fetal bovine serum, 5% chick embryo extract, and antibiotics and plated at 25,000 cells / well in each of the wells of a 96 well plate (150 μl / well). The cells were then placed in a 37% C 5% CO2 incubator and allowed to settle overnight. The cells are cultured as primary cultures for a period of up to 5 days following initi...

example 2

Astroglial Commitment by Human Adipose Tissue-Derived Stromal Cells

[0069] A. Stromal cells are isolated from human adipose tissue according to the methods described above. The cells are cultured as primary cultures for a period of up to 5 days following initial plating in a medium composed of, but not limited to, DMEM (high glucose) media containing 10% fetal bovine serum, 5% chick embryo extract, and antibiotics at 37° C. Cells are harvested by trypsin / EDTA digestion prior to differentiation / implantation.

[0070] Cells are transplanted into the central nervous system of immunodeficient mice or rats. Nude / beige or SCID mice or nude rats are anesthetized in a sealed chamber using 3% halothane in oxygen; anesthesia is maintained by intramuscular injection of 6 mg / kg of xylozine and 60 mg / kg of ketamine. The animals are transferred to a sterotaxic apparatus in a clean field. A 2-to-5-mm incision is made in the scalp 2 mm lateral to the bregma. A burr hole is made in the bone 3 mm later...

example 3

Neuronal Commitment by Human Adipose Tissue-Derived Stromal Cells

Improved Repair and Functional Recovery in a Traumatic Nervous System Injury.

[0073] Stromal cells are isolated from human adipose tissue according of an individual patient for autologous or allogeneic transplantation to a histocompatible recipient according to the methods described above. The cells are cultured as primary cultures for a period of up to 5 days following initial plating in a medium composed of, but not limited to, DMEM (high glucose) media with 1 mM glutamine but without pyruvate, containing 10% fetal bovine serum, 10% newborn calf serum, nucleoside stocks, 0.1 mM 2-mercaptoethanol, 1000 units / ml of leukemia inhibitory factor and antibiotics at 37° C.

[0074] Cells are harvested by trypsin / EDTA digestion prior to differentiation / implantation. Cells are then plated on tissue culture plastic substrate coated with 0.1% sterile gelatin solution. Cells are harvested during rapid growth stage by 0.25% trypsi...

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Abstract

The invention is in the area of pleuripotent stem cells generated from adipose tissue-derived stromal cells and uses thereof. In particular, the invention includes isolated adipose tissue derived stromal cells that have been induced to express at least one phenotypic characteristic of a neuronal, astroglial, hematopoietic progenitor, or hepatic cell. The invention also includes an isolated adipocyte tissue-derived stromal cell that has been dedifferentiated such that there is an absence of adipocyte phenotypic markers.

Description

PRIOR APPLICATION [0001] This application claims priority to U.S. Ser. No. 60 / 185,338, filed on Feb. 26, 2000.FIELD OF THE INVENTION [0002] The invention is in the area of pleuripotent stem cells generated from adipose tissue-derived stromal cells and uses thereof. In particular, the invention includes isolated adipose tissue derived stromal cells that have been induced to express at least one phenotypic characteristic of a neuronal, astroglial, hematopoietic progenitor, or hepatic cell. The invention also includes an isolated adipocyte tissue-derived stromal cell that has been dedifferentiated such that there is an absence of adipocyte phenotypic markers. BACKGROUND OF THE INVENTION [0003] A stem cell must meet the following criteria: (1) ability of a clonal stem cell population to self-renew; (2) ability of a clonal stem cell population to generate a new, terminally differentiated cell type in vitro; (3) Ability of a clonal stem cell population to replace an absent terminally diff...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/30C12N5/08A61K35/12A61K35/28A61K35/407A61K48/00A61P1/16A61P7/00A61P25/00A61P31/00A61P37/00A61P43/00C12N5/071C12N5/0775C12N5/10
CPCA61K35/12A61K48/00C12N5/0618C12N5/0667C12N2506/1384C12N2500/30C12N2500/44C12N2500/80C12N5/067A61P1/16A61P25/00A61P31/00A61P35/00A61P37/00A61P43/00A61P7/00
Inventor WILKISON, WILLIAM O.GIMBLE, JEFFREY
Owner ARTECEL
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