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Microarray having a base cleavable succinate linker

a succinate linker and microarray technology, applied in the field of microarrays, can solve the problems of low yield and reaction conditions that require a relatively long period of tim

Inactive Publication Date: 2007-03-22
CUSTOMARRAY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, formation of an ester linkage to an organic hydroxyl on a solid surface using a succinate is relatively difficult and results in relatively low yield.
Additionally, the reaction conditions require a relatively long period of time at relatively high temperature.

Method used

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  • Microarray having a base cleavable succinate linker
  • Microarray having a base cleavable succinate linker
  • Microarray having a base cleavable succinate linker

Examples

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example 1

Cleavable Linker using Amino Amidite and T-succinate

[0138] In this example, a CombiMatrix CustomArray™ 12K microarray was used to synthesize oligonucleotides attached to the microarray through a base-cleavable linker. The microarray had approximately 12,000 platinum electrodes on a solid surface having a porous reaction layer, wherein each electrode was electronically addressable via computer control. The oligonucleotides were DNA and were synthesized in situ using electrochemical synthesis at locations associated with the electrodes on the microarray. Electrochemical synthesis used standard phosphoramidite chemistry coupled with electrochemical deblocking of the protecting groups on the synthesized DNA for the addition of each nucleotide contained in the oligonucleotide. For attachment of the phosphoramidites, the microarray had organic reactive hydroxyl groups that allowed attachment of the first phosphoramidite. Electrochemical deblocking involved turning on an electrode to gene...

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Abstract

There is disclosed a microarray having base cleavable succinate linkers. The microarray has a solid surface with known locations, each having reactive hydroxyl groups. The density of the known locations is greater than approximately 100 locations per square centimeter. Amino moieties are attached to the reactive hydroxyl groups. Preferably the attachment is through a phosphorous-oxygen bond between the phosphorous of amino amidite moieties and the oxygen of the hydroxyl groups. Succinate moieties are attached to the amino moieties through amide bonds to form cleavable linkers attached to the microarray. Oligomers may be synthesis in situ onto the cleavable linkers and subsequently cleaved using a cleaving base.

Description

TECHNICAL FIELD OF THE INVENTION [0001] This invention provides microarrays having cleavable succinate linker moieties attached at known locations and oligomers synthesized in situ on the cleavable succinate linkers. BACKGROUND OF THE INVENTION [0002] Microarray preparation methods for synthetic oligomers such as oligonucleotides (oligos) include the following: (1) spotting a solution on a prepared flat surface using spotting robots; (2) in situ synthesis by printing reagents via ink jet or other printing technology and using regular phosphoramidite chemistry; (3) in situ parallel synthesis using electrochemically generated acid for deprotection and using regular phosphoramidite chemistry; (4) maskless photo-generated acid (PGA) controlled in situ synthesis and using regular phosphoramidite chemistry; (5) mask-directed in situ parallel synthesis using photo-cleavage of photolabile protecting groups (PLPG); (6) maskless in situ parallel synthesis using PLPG and digital photolithograp...

Claims

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Application Information

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IPC IPC(8): C40B30/06C40B40/04
CPCG01N33/54353B01J19/0046B01J2219/00454B01J2219/00608B01J2219/00653B01J2219/00713B01J2219/00722C12Q1/6837C40B50/18C12Q2525/197C12Q2527/125
Inventor MAURER, KARL
Owner CUSTOMARRAY
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