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Methods and compositions for controlling biofilm development

a technology of compositions and biofilms, applied in the direction of biocide, detergent compounding agents, cleaning compositions, etc., can solve the problems of reducing the life of materials and general harsh treatment to both the plumbing system and the environment, so as to improve the life of materials, inhibit, detach, clean, and/or disperse the effect of biofilms

Inactive Publication Date: 2007-05-17
MONTANA STATE UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides compositions and methods for regulating biofilms. Specifically, the invention involves the use of homoserine lactone blockers or analogs to control, enhance, stimulate, diminish, prevent, inhibit, detach, remove, clean, and / or disperse biofilms. The invention also includes methods for removing biofilm from surfaces to effect cleaning of the surfaces. Overall, the invention offers a novel approach for addressing biofilm-related issues in various fields such as medicine, dentistry, and environmental science.

Problems solved by technology

These biological formations can play a role in restricting or entirely blocking flow in plumbing systems and often decrease the life of materials through corrosive action mediated by the embedded bacteria.
Such treatments are generally harsh to both the plumbing systems and the environment, and have been necessary due to the recalcitrant nature of biofilms within those systems.

Method used

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  • Methods and compositions for controlling biofilm development
  • Methods and compositions for controlling biofilm development
  • Methods and compositions for controlling biofilm development

Examples

Experimental program
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Effect test

example 1

[0106] The most unequivocal experimental design, to determine the role of HSL signal molecules on the formation of biofilms by cells of P. aeruginosa, was to use direct microscopic methods to monitor biofilm formation by cells of HSL negative mutants. For this reason planktonic cells of a wild-type strain (PAO1), and of three mutants incapable of synthesizing specific HSLs, were introduced into flow cells, and adhesion and biofilm formation were monitored by means of confocal scanning laser microscopy (CSLM). Using these techniques, it is possible to monitor the development of live biofilms of the strains of interest.

[0107] Bacteria and media. The Bacteria strains used in this study are listed in Table 1. All experiments were performed using a defined culture medium containing the following, in grams per liter: sodium lactate, 0.05; sodium succinate, 0.05; ammonium nitrate, 0.05; KH2PO4, 0.19; K2HOP4, 0.63; Hutner Salts (Cohen-Bazire, 1957), 0.01; glucose, 1.0; and L-histidine, 0.0...

example 2

[0114] To confirm that HSL was responsible for the architectural differences noted between wild-type and mutant biofilms, an experiment was performed to demonstrate that addition of filterable material collected from medium in which the wild-type organism had grown would recover the wild-type architecture in the double mutant, P. aeruginosa PAO-JP2. When the double mutant was thus grown as a biofilm, it developed an intermediate from between the wild-type and the untreated double mutant. The interior of the cell clusters appeared similar to the untreated P. aeruginosa PAO-JP2 and the exterior of the cell clusters appeared similar to the wild-type organism.

[0115] This experiment was repeated, culturing the double mutant using a concentration of 10 μM OdDHL in fresh medium. This resulted in recovery of the intermediary phenotype as was observed when the cells were grown in the presence of spent medium. These results indicated that biofilm architecture P. aeruqinosa PAO1 biofilms is c...

example 3

Use of OdDHL Analog to Prevent Normal Biofilm Development

[0122] As a typical embodiment of the invention, an OdDHL blocking agent was used to demonstrate that normal biofilm development could be disrupted and the biofilm dispersed following the addition of 0.2% SDS. An analog of OdDHL, N-(2,2-difluorodecanoyl)-L-homoserine lactone was dissolved in 25% ethanol, 75% water by volume and added at a final concentration of 10 uM to growing biofilm cultures of P. aeruginosa PAO1 wild-type. Following 10 days incubation, the biofilm was analyzed for cell to cell distances, cell cluster thickness and response to treatment with 0.2% SDS. It was shown after 10 days incubation that the average cell to cell distance was less than 4 um and was not significantly different from that observed with the OdDHL knockout mutant P. aeruginosa PAO-JP1. The cell cluster average thickness was under 20 um and was not significantly different from the OdDHL knockout P. aeruginosa PAO-JP1. Finally, when treated ...

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Abstract

A method of cleaning or protecting surfaces by treatment with compositions comprising N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) blocking compounds and / or N-butyryl-L-homoserine lactone (BHL) analogs, either in combination or separately.

Description

CROSS-REFERENCE TO RELATED APPLICATION [0001] This application claims the benefit of the filing date of Provisional Application 60 / 050,093, filed Jun. 18, 1997.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT [0002] Not applicable. TECHNICAL FIELD [0003] This invention relates to certain homoserine lactone compositions and methods for their use in biofilm regulation. BACKGROUND OF THE INVENTION [0004] Biofilms are biological films that develop and persist at interfaces in aqueous environments (Geesey, et al., Can. J. Microbiol. 32. 1733-6, 1977; 1994; Boivin and Costerton, Elsevier Appl. Sci., London, 53-62, 1991; Khoury, et al., ASAIO, 38, M174-178, 1992; Costerton, et al., J. Bacteriol., 176, 2137-2142, 1994), especially along the inner walls of conduit material in industrial facilities, in household plumbing systems, on medical implants, or as foci of chronic infections. These biological films are composed of microorganisms embedded in an organic gelatinous structu...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K8/49A61K31/381A61K31/365A01N43/08C02F1/50
CPCA01N43/08A01N61/00A61K8/4973A61K8/69A61K31/365A61K31/381A61L2/18A61L2/22A61L2/23A61Q11/00C02F1/50C11D1/146C11D3/0078C11D3/30C11D3/3481C11D3/48A01N2300/00
Inventor DAVIES, DAVID G.COSTERTON, JOHN WILLIAMPARSEK, MATTHEW R.GREENBERG, E. PETERPEARSON, JAMES P.IGLEWSKI, BARBARA H.
Owner MONTANA STATE UNIVERSITY
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