Compositions and methods for the treatment and repair of defects or lesions in articular cartilage using synovial-derived tissue or cells
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EXAMPLE I
Use of Synovial Membranes to Cover Articular Cartilage Defects In Vivo
[0115] In order to test the effectiveness of using a synovial membrane to cover articular cartilage defects, defects 5 mm wide, 10 mm long and 0.7 mm deep were created with a planing instrument in mature goats. The new defects were filled with a fibrin matrix containing free proliferation agent (IGF-1) at a concentration of 40 ng / ml and a liposome-encapsulated transforming growth factor (BMP-2) at a concentration of 1.0 μg / ml. The defect was then covered with a synovial membrane that was excised from the joint wall, of the same dimensions, and sutured to the defect borders by vycril 7.0 suture material by using single interrupted sutures. After closure of the joint the animals were kept with the joint immobilized in a soft cast over 4 weeks (n=6 animals). Following euthanasia and histological analysis, it was found that the synovial membrane was well incorporated into the surrounding cartilage tissue bo...
Example
EXAMPLE II. A
Use of Synovial Bits to Repair Articular Cartilage Defects In Vivo
[0116] In large articular cartilage defects, the process of synovial cell migration from the synovium into the articular cartilage defect to populate the defect with cells that can be transformed into chondrocytes to repair the cartilage may be too slow or provide insufficient numbers of cells to achieve complete filling by cell proliferation and tissue differentiation within the first few weeks following surgery. To provide a greater number of sources of cells for repair, synovial membrane material was cut into small tissue bits and mixed into a fibrin matrix and deposited, together with a transforming factor, within a defect. The defect was then covered by a synovial covering membrane, as described in the Example I above. All aspects of the experiment were as described above except for the addition of synovial bits to the fibrin matrix. Upon sacrifice of the animal, numerous areas of tissue transforma...
Example
EXAMPLE III
Use of Stacks of Synovial Membranes to Repair Articular Cartilage Defects In Vivo
[0118] The experiment described in Example I. (above) was modified such that the defect was filled with stacks of synovial membranes of the approximate dimensions of the defect itself. Prior to placement in the defect, each of the synovial membranes was soaked in a BMP-2 solution at a concentration of 4.4 mg / ml, to induce transformation into cartilage tissue. Additionally, between the layers of synovial membranes, a small amount of fibrin matrix or microspheres containing transforming factors (BMP-2, 4.4 mg / ml) was deposited to allow for the controlled release of the transforming factor. Macroscopic results showed transformation of the synovial tissue into cartilage-like tissue.
EXAMPLE IV
Use of Cultured Synovial Cells to Repair Articular Cartilage Defects In Vivo
[0119] Treatment of defects similar to those described in Example I (above) can be conducted such that following a surgical int...
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