Smokeable product with reduced content of pathogens
a technology of pathogens and smokeable products, applied in the field of smokeable products, can solve the problems of many naturally occurring tobacco microorganisms being killed, little or no clear health benefits, and the precise way in which inadvertent consumption leads to disease, and achieve the effect of reducing the content of bacteria
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example 1
[0045]Finished cigarettes purchased at retail (Player's Navy Cut Plain, John Player & Sons, Montreal Canada) were sealed in a polypropylene jar and subjected to 5 kGy of ionizing radiation from a Cobalt 60 source. Tobacco was taken from several of the irradiated cigarettes and shaken with distilled water. No culture could be grown at 25° C.±5° C. from samples of this water, neither under aerobic nor anaerobic conditions. Further, drops of this water had no killing effect on multi-species cultures which resulted from using non-irradiated cigarette tobacco as an innoculant. Untreated tobacco from an identical package of cigarettes, cultured identically, was used as a positive control, while pure distilled water was used as a negative control. The positive control resulted in irregular multi-culture growths with occasional clear plaque spots; the negative control remained clear.
example 2
[0046]Finished cigars purchased at retail (Century Sam) were sealed in a polypropylene jar and subjected to 5 kGy of ionizing radiation from a Cobalt 60 source. Tobacco, including wrapper tobacco leaves and cut filler tobacco, was taken from one of the irradiated cigars and shaken with distilled water. No culture could be grown at 25° C.±5° C. from samples of this water, neither under aerobic nor anaerobic conditions. Further, drops of this water had no killing effect on multi-species cultures which resulted from using non-irradiated cigar tobacco as an innoculant. Untreated tobacco from a cigar of the same brand, purchased at the same time and cultured identically, was used as a positive control. The positive control resulted in irregular multi-culture growths; the negative control remained clear.
[0047]It is clear that the radiation treatment had the effect of very greatly reducing the content of living species, and the polypropylene jar was clearly effective in preventing re-conta...
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