Adipose Tissue Stem Cells, Perivascular Cells and Pericytes

a technology of perivascular cells and stem cells, applied in the field of adipose tissue stem cells, perivascular cells and pericytes derived from adipose tissue, can solve the problems of affecting the use of such cells, affecting the quality of bone marrow, and affecting the ability of bone marrow to be harvested, so as to increase vascularity and enhance angiogenesis

Inactive Publication Date: 2008-09-04
UNIV OF VIRGINIA ALUMNI PATENTS FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0017]Applicants have found that human Adipose Stem Cells (ASCs) can enhance angiogenesis and increase vascularity in in vivo models of inflammation and/or tissue injury.
[0018]While prior art and/or other researchers have d...

Problems solved by technology

While the identification of such cells has led to advances in tissue regrowth and differentiation, the use of such cells is hampered by several technical hurdles.
One drawback to the use of such cells is that they are very rare (representing as few as 1/2,000,000 cells), making any process for obtaining and isolating them difficult and costly.
Of course, bone marrow harvest is universally painful to the donor.
Moreover, such cells are difficult to culture without inducing differentiati...

Method used

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  • Adipose Tissue Stem Cells, Perivascular Cells and Pericytes
  • Adipose Tissue Stem Cells, Perivascular Cells and Pericytes
  • Adipose Tissue Stem Cells, Perivascular Cells and Pericytes

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embodiments

[0130]The present invention provides a perivascular cell, or pericyte, derived from adipose tissue. In one aspect, the cell is a precursor to a perivascular cell. The phenotype can be determined, inter alia, by positional fate, morphological characteristics, and other phenotypic characteristics such as cell-surface markers and biological functional characteristics such as increased microvascular density.

[0131]In one embodiment, the invention provides methods of identifying such cells and their precursors. In another embodiment, the invention provides methods of enriching populations of such cells. In one aspect, the methods of enriching such cells includes methods of inducing differentiation of the precursor cells.

[0132]In one embodiment, the present invention provides methods of increasing vascularity / vascular density in vivo using ASC derived perivascular precursor cell subpopulations. Such subpopulations may be derived from freshly derived adipose tissue or from cultured adipose ...

examples

[0222]The studies described herein investigate, at the single cell level, the potential of hASCs to contribute to microvascular growth when injected in vivo, and we have found that injected hASCs migrate to the abluminal surface of microvessels and alter their cell morphology to conform to the curvature of the microvessel in a manner that is consistent with perivascular (and not endothelial) cell behavior. Also examined herein is the expression of various perivascular cell markers, focusing on the pericyte population, a cell population defined by their morphological structure around microvessels with cell processes extending beneath the basement membrane. Since pericytes have been suggested to contribute to microvessel growth and maintenance, it was determined whether hASCs function as microvascular support cells by analyzing their perivascular investment in relation to changes in total vascular density. The present application discloses a new role for human adipose-derived stromal ...

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Abstract

The present invention provides methods for growing and inducing perivascular cell differentiation of adipose tissue-derived stromal cells. The invention further provides methods for administering such adipose tissue-derived cells to a subject. The cells of the invention are useful for treating diseases, disorders, conditions, and injuries requiring new or enhanced angiogenesis, vascular remodeling, drug delivery, and tissue engineering.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority under 35 U.S.C. §119(e) to U.S. Provisional Application Ser. No. 60 / 703,591 filed Jul. 29, 2005, the disclosure of which is incorporated by reference in its entirety herein.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT[0002]This invention was made with United States Government support under National Institutes of Health Grant Nos. HL-52309, HL-65958, and HL-721415. The United States Government may therefore have certain rights in the invention.FIELD OF THE INVENTION[0003]The present invention relates generally to adipose tissue-derived stromal cells, and to perivascular cells and pericytes derived from adipose tissue, as well as their use.BACKGROUND[0004]In recent years, the identification of mesenchymal stem cells, chiefly obtained from bone marrow, has led to advances in tissue regrowth and differentiation. Such cells are pluripotent cells found in bone marrow and periosteum, and they a...

Claims

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Application Information

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IPC IPC(8): A61K35/12C12N5/06A61P9/00C12N5/0775
CPCA61K35/12C12N5/0667A61K48/00A61P9/00
Inventor KATZ, ADAM J.COTTLER, SHAYN PEIRCE
Owner UNIV OF VIRGINIA ALUMNI PATENTS FOUND
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