High Pressure Processing of Bioactive Compositions

a bioactive composition and high pressure technology, applied in the direction of transferrins, immune disorders, extracellular fluid disorders, etc., can solve the problems of thermal processing not generally suitable for the production of commercially sterile bioactive products, the like, and affecting the bioactivity of food products

Inactive Publication Date: 2008-12-25
FONTERRA COOP GRP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional means of ensuring a useful keeping quality have a negative impact on the bioactivity of food products and the like.
In particular, thermal processing is not generally suitable for the production of commercially sterile bioactive products.
There are many processes in the manufacture of bioactive products, ingredients and foods that may result in a partial or complete loss of bioactivity.
Drying of products produced using pasteurised milk may be used to improve keeping quality with losses of up to 40% of immunoglobulins (Li-Chan, 1995), but commercial applications are then limited to direct consumption (for example, tablets) or fresh products (for example, yogurt) where the dried bioactive ingredient is not subsequently heated again.
Losses due to drying and heating may be compensated for by supplementing intermediate or final products with the bioactive component of interest but this can increase the cost to the end consumer.
However, Huppertz et al (2002) report that high pressure denatures whey proteins in milk.
Additionally, Korhonen et al (1998) report that pressure treatments at pressures of about 500 MPa and above irreversibly denature proteins in most cases.
Masuda et al (2000) report that pressures of 400 MPa and above may not be used to improve the keeping quality of bovine colostrum because such pressures denature the immunoglobulin protein.

Method used

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  • High Pressure Processing of Bioactive Compositions
  • High Pressure Processing of Bioactive Compositions
  • High Pressure Processing of Bioactive Compositions

Examples

Experimental program
Comparison scheme
Effect test

example 1

Solution Containing Colostrum Ingredient

[0205]A colostrum milk protein concentrate powder (Fonterra Co-operative Group Limited) containing 80% protein (and 6.6% immunoglobulins) was made up with water and the pH adjusted to 3.5 with lactic acid to yield a 3.6% protein solution. A sample of the solution was then heat-treated or pressure-treated at 83.5° C. or 600 MPa respectively, held for 3 minutes, and the quantity of immunoglobulins in the treated beverages was measured by HPLC and compared to the quantity in the unprocessed solution. The results are shown in FIG. 1. An HPP unit from Stansted Fluid Power Ltd, UK was used for all examples.

example 2

Solutions Containing Colostrum MPC Ingredient

[0206]Colostrum MPC was dissolved in a 0.3% w / v pectin solution to prepare a 6% w / v stock solution of colostrum MPC. Samples of the colostrum stock solution were acidified to a range of pH values and either heat-processed at 85° C. and held for 10 min or pressure-processed as described below. The residual immunoglobulin G (IgG) of each heat- and pressure-processed sample relative to an unprocessed control at the same pH was measured by HPLC-MC. The solutions were also challenged with coliforms, yeast and mould and were enumerated for these organisms following pressure treatment at 600 MPa.

[0207]At pH 3.3, a colostrum sample pressure-processed at 400 MPa and held for 3 min had 67% IgG remaining, compared to 2% for heat-processed colostrum.

[0208]At pH 4.1, a colostrum sample pressure-processed at 600 MPa and held for 3 min had 37% IgG remaining, compared to 2% for heat-processed colostrum.

[0209]At pH 3.5, a colostrum sample pressure-process...

example 3

Colostrum MPC Ingredient at Various pH

[0213]Colostrum samples from the stock solution of Example 2 were acidified to pH 3.5 to 4.1 and pressure processed at 400 to 600 MPa (no hold) and compared to a heat-processed preparation. In all cases approximately 2% residual IgG was retained after heat treatment, compared to 45-100% residual IgG retained after pressure treatment, with the highest residual IgG at the higher pH. The results are summarised in Table 2.

TABLE 2Residual IgG (%) of heat-or pressure-processed6% w / v colostrum MPCPressurepH 3.5pH 3.8pH 4.1Unprocessed10010010085° C. / 10 min222400 MPa / no hold7592100500 MPa / no hold547391600 MPa / no hold455648

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PUM

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Abstract

The present invention relates to a method of pressure treating a bioactive composition comprising at least one bioactive component to prevent the growth of at least one unwanted microorganism while retaining a desired level activity of the at least one bioactive component. The bioactive component is selected from one or more proteins protein hydrolysates, one or more lipids or lipid hydrolysates, one or more carbohydrates, one or more probiotic factors, or mixtures thereof. The pressure treatment is at a predetermined pressure from about 350 to 1000 MPa.

Description

FIELD OF INVENTION[0001]The present invention relates to the high pressure processing of bioactive compositions and in particular to a method of pressure treating a bioactive composition to prevent the growth of at least one unwanted microorganism while retaining a desired level of activity of at least one bioactive component.BACKGROUND[0002]The delivery of bioactive components (proteins, lipids or hydrolysates thereof, and probiotic microorganisms, for example) in food or other ingestible products is constrained by the need to provide a safe product with a useful shelf life while retaining bioactivity. Products with a useful shelf life are said to have a good keeping quality and are less prone to spoilage.[0003]Delivery of bioactive components is desirable at least because such components are physiologically active when ingested and can have positive health benefits, including but not limited to bone health, immune benefits, anti-inflammatory activity, heart health and efficacy in ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K47/46A01N63/50A23L5/30A23L21/10A23L29/20
CPCA01N63/02A23C3/00A23C3/08A23C9/1322A23C2210/15A23L2/66A23L3/0155A23V2002/00A61K35/20A61L2/0011C07K14/79A23V2200/308A23V2200/306A23V2200/324A23V2250/54248A23L5/30A23L33/165A23L33/19A61P19/00A61P19/02A61P19/10A61P29/00A61P35/00A61P37/02A61P37/04A61P7/06A61P9/00A01N63/50A01N63/10A23L3/015
Inventor CARROLL, TIMOTHY JOSEPHPATEL, HASMUKH AMBALALGONZALEZ-MARTIN, MIGUEL ALEJANDRODEKKER, JAMES WILLIAMCOLLETT, MICHAEL ANTHONYLUBBERS, MARC WILLIAM
Owner FONTERRA COOP GRP LTD
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