Light activated gene transduction using long wavelength ultraviolet light for cell targeted gene delivery

Inactive Publication Date: 2009-04-09
SCHWARZ EDWARD M +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014]A feature of certain preferred embodiments of this invention is the avoidance of the problems involved with using UV and γ-irradiation through the use of locally administered, long wavelength UV (i.e.,

Problems solved by technology

The introduction of either the co-infection or the DNA damaging agents dramatically induces the rate limiting step of second strand synthesis, i.e. the second strand of DNA which is synthesized based on the vector inserted first strand.
However, making use of these DNA damaging agents is impractical because the administration of an adenovirus co-infection to a patient is not practical or desirable and the site specific and sa

Method used

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  • Light activated gene transduction using long wavelength ultraviolet light for cell targeted gene delivery
  • Light activated gene transduction using long wavelength ultraviolet light for cell targeted gene delivery
  • Light activated gene transduction using long wavelength ultraviolet light for cell targeted gene delivery

Examples

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example 1

I. Methods

[0052]A. Isolation of Human Mesenchymal Stem Cells

[0053]Human Mesenchymal Stem Cells (hMSC) were isolated from patient blood samples harvested from the iliac crest. The blood samples were diluted in an equal volume of sterile Phosphate Buffered Saline (PBS). The diluted sample was then gently layered over 10 ml of Lymphoprep (Media Prep) in a 50 ml conical tube (Corning). The samples were then centrifuged at 1800 rpm for 30 minutes. This isolation protocol is a standard laboratory technique, and the resulting gradient that formed enabled the isolation of the hMSCs from the layer immediately above the Lymphoprep. The isolated fraction was placed into a new 50 ml conical tube, along with an additional 20 ml of sterile PBS. The sample was centrifuged at 1400 rpm for 8 minutes. The supernatant was removed the cell pellet was resuspended in 20 ml for fresh PBS, and centrifuged again for 8 minutes at 1400 rpm. Afterwards the supernatant was removed, the cell pellet was resuspend...

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Abstract

In accordance with the present invention, methods are provided for treating a patient through the use of ultraviolet light activated gene therapy. Embodiments of the present invention include methods for the utilization of light activated gene therapy to repair and/or rebuild damaged cartilage by introducing a desired gene into a patient's tissue.

Description

REFERENCE TO RELATED APPLICATIONS[0001]The present application is a continuation of U.S. application Ser. No. 10 / 357,271, filed Jan. 31, 2003, the entirety of which is incorporated herein by reference. U.S. application Ser. No. 10 / 357,271, and the present application through U.S. application Ser. No. 10 / 357,271, claim the priority benefit under 35 U.S.C. §119(e) of Provisional Application No. 60 / 353,842, filed on Jan. 31, 2002. The present application is also related to Provisional Application No. 60 / 353,907, filed on Jan. 31, 2002, and U.S. application Ser. No. 10 / 357,273, filed on Jan. 31, 2003.GOVERNMENT INTEREST[0002]This invention was made with Government support under NIH Contract #AR45972, an RO1 grant awarded by NIAMS. The Government has certain rights in the invention.BACKGROUND OF THE INVENTION[0003]1. Field of the Invention[0004]The invention relates generally to the field of gene therapy. According to the present invention, devices and methods are provided for the combin...

Claims

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Application Information

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IPC IPC(8): A61N5/06A61M31/00A61K48/00C07H21/04C12N15/864
CPCA61K48/00A61K48/0008C12N2750/14143C12N15/86A61K48/0083
Inventor SCHWARZ, EDWARD M.O'KEEFE, REGIS J.FOSTER, THOMASFINLAY, JAROD C.
Owner SCHWARZ EDWARD M
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