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Methods and collagen products for tissue repair

a collagen product and tissue repair technology, applied in the direction of peptide/protein ingredients, ligaments, prostheses, etc., can solve the problems of premature loss of fibrin clot scaffold, meniscus and articular cartilage in human joints that fail to heal after an injury, and disrupt the healing process of tissues within the joint or intra-articular tissues

Inactive Publication Date: 2009-10-08
CHILDRENS MEDICAL CENT CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0028]The invention according to other aspects is a method comprising contacting the ends of a ruptured articular tissue in a subject with a sterile solution of solubilized collagen in a concentration of greater than 5 and less than or equal to 50 mg / ml and having a viscosity of 1,000-200,000 centipoises and a pH of 6.8-8.0, and hydroxyproline in a concentration of 0.1-5.0 μg / ml, wherein the solution has an osmolarity of 280-350 mOs / kg, wherein the composition does not include thrombin, and allowing the solution to set to treat the ruptured articular tissue.

Problems solved by technology

In addition, the meniscus and the articular cartilage in human joints also often fail to heal after an injury.
This fibrinolytic process results in premature loss of the fibrin clot scaffold and disruption of the healing process for tissues within the joint or within intra-articular tissues.
These studies have shown some additive effects of applied combinations of growth factors on the wound healing of ligaments; however, even with advanced application techniques, the combinations of growth factors, timing of release and concentration of release make optimization of these systems difficult.

Method used

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  • Methods and collagen products for tissue repair
  • Methods and collagen products for tissue repair
  • Methods and collagen products for tissue repair

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Collagen solution and testing of Properties

[0160]A. Minimal Gelation Achieved with Some Formulations

[0161]1. Innocoll: Aliquots of Innocoll Collagen (Starting pH=4.1) were Made.

[0162]Weights of aliquots were 0.380 mg collagen, pH=4.1. One half of the samples had 5 microliters NaHCO3 added to bring pH to between 7.0 and 8.0 and one half were not neutralized. 300 microliters fetal bovine serum was added to each aliquot. All solutions were monitored for gelation at 37° C. for 30 minutes. The solutions remained liquid, with viscosities similar to that of water (approximately 1 centipoise). No increase in viscosity with time was noted over the hour long period.

[0163]Identical experiments with Innocoll aliquots at starting pH=2.5 were also performed. Additionally, experiments were performed using ratios of collagen:FBS of 1:1, 2:1 and 3:1. None of these materials produced a gelled product at 37° C. The solutions remained liquid, with viscosities similar to that of water (ap...

example 2

[0194]In this Example, type I collagen was used to stimulate activation of the fibrin clotting mechanism and platelet activation. Initially, we tested collagen to determine if it would result in more sustained release of two growth factors used as markers of platelet function, namely TGF-β1 and PDGF-αβ, when compared with the use of exogenous thrombin. Secondly, we tested whether the amount of this release would be dependent on the platelet concentration in the PRP. The release profiles of these growth factors from three types of collagen-PRP gels were compared with the release profile from a PRP clot created with exogenous bovine thrombin over 10 days. Additionally, we determined whether the growth factor release from collagen-activated PRP hydrogels would cause a physiologic changes in ACL cells in terms of 1) cellular metabolism of growth factors, 2) cellular proliferation within the gels and 3) cell-mediated gel contraction.

Materials and Methods

Preparation of Platelet-Rich Plasm...

example 3

[0231]The components of a preferred collagen solution of the invention was tested to identify components.

Methods

[0232]Collagen from various sources (Cellagen, MP Biomedicals, Solon, Ohio (shown as lane 4 in FIG. 11); Elastin Products Company, Inc., Owensville, Mo. (shown as lane 5 in FIG. 11); StemCell Technologies (shown as lane 6 in FIG. 11), Becton Dickinson, Franklin Lakes, N.J. (shown as lane 7 in FIG. 11); fluorescein-labeled collagen (shown as lane 8 in FIG. 11)) were prepared into aliquot samples. Total protein content of each collagen sample was determined using a calorimetric assay (BCA Protein Assay Kit, Rockford, Ill.) in order to aliquot samples containing equal protein content. The aliquots were treated with SDS and β-mercaptoethanol and placed at 100° C. for five minutes for denaturation and then loaded onto a 4-12% SDS-PAGE gel. The gels were stained with Coomassie Blue (Bio-Rad, Hercules, Calif.) and washed with a 7.5% acetic acid and 5% methanol destaining solution...

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Abstract

Methods and devices for the repair of articular tissue using collagen material are provided. Compositions of collagen material and related kits are also provided.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a Continuation Application of co-pending International Application PCT / US2007 / 021009 filed Sep. 28, 2007, which designated the U.S., and claims benefit under 35 USC 119(e) of U.S. Ser. No. 60 / 847,743 filed on Sep. 28, 2006, the contents of which are herein incorporated by reference in their entirety.FEDERALLY SPONSORED RESEARCH[0002]This invention was made with Government support under NIH K02 AR049346. Accordingly, the Government may have certain rights in this invention.FIELD OF THE INVENTION[0003]The invention relates generally to methods and devices for the repair of articular tissue using collagen materials.BACKGROUND OF THE INVENTION[0004]Intra-articular tissues, such as the anterior cruciate ligament (ACL), do not heal after rupture. In addition, the meniscus and the articular cartilage in human joints also often fail to heal after an injury. Tissues found outside of joints heal by forming a fibrin clot, which c...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61B17/08A61F2/00
CPCA61K38/39A61K35/19A61L27/24A61L27/54A61L27/56A61L27/58A61L2300/252A61L2300/406A61L2300/432A61L2300/602A61K45/06A61K35/17A61K31/401A61K2300/00A61P19/02A61F2/0811A61L27/3616A61L27/3662A61L27/50A61L2300/64A61L2400/06A61L2430/10
Inventor MURRAY, MARTHA M.
Owner CHILDRENS MEDICAL CENT CORP
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