Powders for reconstitution
a technology of reconstitution and powder, which is applied in the direction of biocide, drug composition, dispersed delivery, etc., can solve the problems of resistance, no currently available drug therapy is capable of completely eradicating hiv, and emergence of resistant mutants
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example 1
[0069]4 g TMC278 base and 36 g PVP-VA 64 are dissolved in 800 ml of a mixture of methanol / methylene chloride 50:50 (v / v). This mixture is spray dried at 80° C. (inlet temperature) using a Mini Spray Dryer B-191 (Büchi, Switzerland). The flow control was set at 800 N 1 / h pressurized air, the aspirator at 100% and the pump at 50%. Afterwards, the resulting powders were post dried in a vacuum dryer (Christ Alpha, Medizinischer Apparatebau, OsterodelHarz, Germany) for 44 hours.
example 2
[0070]4 g TMC278 base, 34 g PVP-VA 64 and 2 g Cremophor EL are dissolved in 800 ml of a mixture of methanol / methylene chloride 50:50 (v / v). This mixture is the spray dried at 80° C. (inlet temperature) using a Mini Spray Dryer B-191 (Büchi, Switzerland). The flow control was set at 800 N 1 / h pressurized air, the aspirator at 100% and the pump at 50%. Afterwards, the resulting powders were post dried in a vacuum dryer (Christ Alpha, as above) for 44 hours.
[0071]Dissolution:
[0072]The powders for reconstitution of example 1 and 2 were tested on dissolution. During the dissolution test, the powders for reconstitution of TMC278 was suspended into 50 ml of water and the resulting suspension was added into 500 ml of the 0.01 M HCl aqueous solution serving as dissolution medium resulting in a final suspension volume of 550 ml. At each time point, 5.0 ml of the final suspension was removed and the volume was compensated by adding 5.0 ml of fresh dissolution medium. So, to calculate the % of ...
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