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Blue transgenic fluorescent ornamental fish

a transgenic, fluorescent technology, applied in the field of transgenic fish, can solve the problems of limited progress in the field of ornamental fish that express such proteins, and the striped pigmentation of adult zebrafish does not aid in the efficient display of various colors

Active Publication Date: 2012-08-16
GLOFISH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention relates to making transgenic fish and providing them to the ornamental fish industry. Specifically, the invention provides methods for creating fertile, transgenic fish with specific transformation events that result in aesthetically pleasing blue color. The fish can be homozygous or heterozygous for the transformation event, and the offspring can be produced using in vitro fertilization techniques. The use of sperm with the transformation event can also be used to create transgenic offspring. The invention also includes methods for producing transgenic organisms using the transformed fish sperm. Overall, the invention provides a way to create desirable fish with specific color patterns for the ornamental fish market.

Problems solved by technology

However, for the ornamental fish industry the dark striped pigmentation of the adult zebrafish does not aid in the efficient display of the various colors that are currently available on the market.
Although transgenic experiments involving fluorescent proteins have provided new markers and reporters for transgenesis, progress in the field of developing and producing ornamental fish that express such proteins has been limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Blue Transgenic Zebrafish

[0029]Transgenic fish exhibiting a blue color are provided. The specific transgenic events embodied in these fish are designated Blue zebrafish 1. Sperm from these fish may be used to fertilize zebrafish eggs and thereby breed transgenic zebrafish that comprise these specific transgenic integration events. Sperm from this line is deposited at the European Collection of Cell Cultures (ECACC) as “Blue zebrafish 1” (accession no. 11012802; cell line ZEBRAFISH 2011.2 BZF001).

[0030]The fluorescent transgenic fish have use as ornamental fish in the market. Stably expressing transgenic lines can be developed by breeding a transgenic individual with a wild-type fish, mutant fish, or another transgenic fish. The desired transgenic fish can be distinguished from non-transgenic fish by observing the fish in white light, sunlight, ultraviolet light, blue light, or any other useful lighting condition that allows visualization of the blue color of the transgenic fish.

[003...

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Abstract

The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Description

[0001]This application claims priority to U.S. Provisional Application No. 61 / 443,070 filed on Feb. 15, 2011, the entire contents of which are specifically incorporated herein by reference without disclaimer.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]This invention relates to transgenic fish, particularly blue transgenic fish.[0004]2. Description of Related Art[0005]Transgenic technology involves the transfer of a foreign gene into a host organism enabling the host to acquire a new and inheritable trait. Transgenic technology has many potential applications. For example, it can be used to introduce a transgene into a fish in order to create new varieties of fish. There are many ways of introducing a foreign gene into fish, including: microinjection (e.g., Zhu et al., 1985; Du et al., 1992), electroporation (Powers et al., 1992), sperm-mediated gene transfer (Khoo et al., 1992; Sin et al., 1993), gene bombardment or gene gun (Zelenin et al., 1991), liposome-media...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01K67/027A01K61/00A01K67/02
CPCA01K67/0275A01K2267/02A01K2217/052
Inventor BLAKE, ALANCROCKETT, RICHARDNASEVICIUS, AIDAS
Owner GLOFISH LLC
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