Blue transgenic fluorescent ornamental fish

Abstract

The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Description

[0001]This application claims priority to U.S. Provisional Application No. 61 / 443,070 filed on Feb. 15, 2011, the entire contents of which are specifically incorporated herein by reference without disclaimer.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]This invention relates to transgenic fish, particularly blue transgenic fish.[0004]2. Description of Related Art[0005]Transgenic technology involves the transfer of a foreign gene into a host organism enabling the host to acquire a new and inheritable trait. Transgenic technology has many potential applications. For example, it can be used to introduce a transgene into a fish in order to create new varieties of fish. There are many ways of introducing a foreign gene into fish, including: microinjection (e.g., Zhu et al., 1985; Du et al., 1992), electroporation (Powers et al., 1992), sperm-mediated gene transfer (Khoo et al., 1992; Sin et al., 1993), gene bombardment or gene gun (Zelenin et al., 1991), liposome-media...

AI Technical Summary

Problems solved by technology

However, for the ornamental fish industry the dark striped pigmentation of the adult zebrafish does not aid in the efficient display of the various colors that are currently available on the market.

Although transgenic experiments involving fluorescent proteins have provided new markers and reporters for transgenesis, progress in the field of developing and producing ornamental fish that express such proteins has been limited.