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Oral composition

a technology of oral composition and composition, applied in the field of oral composition, can solve the problems of resistance bacteria emergence risk, difficult to practice suitable oral care, and failure to achieve the intended effect of conventional periodontal disease prevention, and achieve the effect of reducing the risk of resistant bacteria emergen

Inactive Publication Date: 2014-08-14
LOTTE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention introduces a new approach to preventing periodontal disease. Compared to existing antibacterial agents, this approach is believed to have a lower risk of developing resistant bacteria. It is believed that this new approach will be more effective in preventing periodontal disease.

Problems solved by technology

Therefore, the conventional periodontal disease prevention fails to achieve an intended effect, and additionally always facing the risk of the resistant bacteria emergence.
Also, mechanical removal such as brushing, and scaling, is believed to be the most effective method for inhibiting a biofilm, but it is difficult to practice suitable oral care using the current procedure for people having difficulties in such a mechanical control of dental biofilm such as the elderly in need of nursing care, whereby the development of biofilm removal procedures different from the convention procedures are in demand.
However, PTL 1 does not disclose nor suggest any effects on the periodontal diseases.

Method used

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  • Oral composition

Examples

Experimental program
Comparison scheme
Effect test

example

Example 1

[0023](Extract Preparation)

[0024]Each of the plant samples was a commercially purchased product. After freeze-dried, 1 g of each of the plant samples was finely ground using a grinder, and extracted with 50 ml of water at 70° C. for 2 hours. The obtained extract was centrifuged at 3000 rpm for 10 minutes, the supernatant thereof was filtered and freeze-dried to be tested as a hot water extract of each plant.

example 2

[0025](Evaluation on the Biofilm Formation Inhibitory Activity)

[0026]1. Biofilm Formation

[0027]A. naeslundii ATCC19039 strain was anaerobically cultured in 5 ml of Brain Heart Infusion (BHI) liquid media at 37° C. for 10 hours. Then, the bacteria collected by the centrifugal separation at 3000 rpm for 10 minutes was treated with PBS to adjust to OD550 nm=0.5 and used as a test sample suspension.

[0028]The test of biofilm formation was carried out using a 96-well microplate. In each well, 60 μl of the biofilm formation inhibitory sample, 20 μl of butyric acid, 20 μl of a test sample suspension containing A. naeslundiiand 100 μl of Toripticase soy broth with 0.5% sucrose added thereto were added and incubated for 16 hours at 37° C. under the conditions of 5% CO2.

[0029]2. Quantitative Determination of Biofilm

[0030]The supernatant after the above incubation was removed and each well was washed twice with PBS. After washing, a 0.25% safranine solution was added to each well, allowed to st...

example 3

[0045]A mouthwash was produced according to the following formulation.

Ethanol2.0% by weightJapanese mustard spinach extract1.0Flavor1.0WaterBalance100.0

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Abstract

An oral composition having a biofilm formation inhibitory action is provided. An oral composition, comprising a plant extract which is a hot water extract of Japanese mustard spinach, potherb mustard, mibuna, hot radish, cress, ostrich fern, Japanese angelica tree, or ice plant.

Description

TECHNICAL FIELD[0001]The present invention relates to an oral composition having an inhibitory action by a plant extract on Actinomyces naeslundii (hereinafter referred to as “A. naeslundii”) biofilm formation.BACKGROUND ART[0002]Microorganisms attached on the surface of an object do not exist independently, but form a biofilm together with other microorganisms in the characteristic structure. The biofilm, which appears to work in favor of the human as seen in the use of immobilized microorganisms, is conversely revealed to cause tooth decay and food contamination, and has been extensively studied in recent years.[0003]The dental biofilm includes 700 species or more of bacteria and 108 or more bacteria are present per 1 mg of the dental biofilm. Of these, A. naeslundii has been receiving much attention in recent years as a key microorganism required when the initial stage plaque (cariogenic plaque) proceeds to the late stage plaque (periodontal disease plaque).[0004]In the conventio...

Claims

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Application Information

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IPC IPC(8): A61K8/97A23L1/30A61Q11/00
CPCA61K8/97A23L1/3002A61Q11/00A23G3/48A23G4/068A23L2/02A23L2/52A23L33/105A61K8/9789A61K36/11A61K36/185A61K36/25A61K36/31A61P1/02A61K2300/00A61K2236/331A23V2002/00A61K8/0204A61K8/0216A61K9/0056A61K9/0058A61K9/007A61K9/0095A61K9/20A61K2236/33A61K2800/92
Inventor TSUGANE, TAKANORIMOHRI, SHOTASAEKI, YOJI
Owner LOTTE CO LTD