Anticancer composition containing mixed herbal medicine extract as active ingredient

Inactive Publication Date: 2016-08-25
UNIV IND COOP GRP OF KYUNG HEE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014]The mixed extract of Astragalus membranaceus, Angelica gigas, and Trichosanthes kirilowii according to the present invention does not impact normal cell growth, while having an anticancer effect which spe

Problems solved by technology

A carcinogen which is the cancer initiator includes smoking, ultraviolet rays, chemicals, foods and other environmental factors, but the initiator is various and thus, it is difficult to develop medicines and effects of the medicines vary according to a generated site.
Currentl

Method used

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  • Anticancer composition containing mixed herbal medicine extract as active ingredient
  • Anticancer composition containing mixed herbal medicine extract as active ingredient
  • Anticancer composition containing mixed herbal medicine extract as active ingredient

Examples

Experimental program
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Effect test

example 1

Preparation of Mixed Extract of Astragalus membranaceus, Angelica Gigas, and Trichosanthes kirilowii

[0068]Astragalus membranaceus (Am), Angelica gigas (Ag), and Trichosanthes kirilowii Maximowicz (Tk) were mixed with a weight ratio (w / w) of (1) 1:1:1 or (2) 3:1:1, respectively, put in an extractor, and extracted for 2 to 3 hours after adding 8 to 10 times water and 30% (v / v) ethanol or 80% ethanol, respectively. The extract was filtered and the filtrate was dried after decompression-concentrating to obtain a mixed extract (a water extract, a 30% ethanol extract, and a 80% ethanol extract) of Astragalus membranaceus, Angelica gigas, and Trichosanthes kirilowii (about 35.5% average yield of the mixed extract mixed with a weight ratio of 1:1:1 and about 29.85% average yield of the mixed extract mixed with a weight ratio of 3:1:1).

example 2

Preparation of Mixed Extract of Astragalus membranaceus, Angelica Gigas, Trichosanthes Kirilowii, and Ginseng

[0069]Astragalus membranaceus, Angelica gigas, Trichosanthes kirilowii Maximowicz, and ginseng were mixed with a weight ratio (w / w) of (1) 1:1:1:1 or (2) 3:1:1:1, respectively, put in an extractor, and extracted for 2 to 3 hours after adding 8 to 10 times water and 30% (v / v) ethanol or 80% ethanol, respectively. The extract was filtered and the filtrate was dried after decompression-concentrating to obtain a mixed extract (a water extract, a 30% ethanol extract, and a 80% ethanol extract) of Astragalus membranaceus, Angelica gigas, Trichosanthes kirilowii, and ginseng (about 28% average yield of the mixed extract mixed with a weight ratio of 1:1:1:1 and about 24.24% average yield of the mixed extract mixed with a weight ratio of 3:1:1:1).

experimental example 1

Verification of Effect of Mixed Extract of the Present Invention on Growth of Cancer Cells According to Extractant

[0070]In order to verify an effect of the mixed extracts prepared in Examples 1 and 2 on a growth of cancer cells according to an extractant, an MTT assay was performed by a known method in the related art. A cancer cell line used an MDA-MB-231 breast cancer cell line.

[0071]In more detail, in the MDA-MB-231 breast cancer cell line cultured on a 96-well plate, the mixed extract of Astragalus membranaceus, Angelica gigas, and Trichosanthes kirilowii or the mixed extract of Astragalus membranaceus, Angelica gigas, Trichosanthes kirilowii, and ginseng which was prepared in Examples 1 and 2 was treated with various concentrations (50 to 500 μg / ml) and then cultured for 48 hours under a condition of 37° C. and 5% CO2. Thereafter, a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was treated in each cell, the cells were further cultured for 4 hours, a...

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Abstract

An embodiments of the invention relates to a composition containing, as an active ingredient, a mixed extract of Astragalus membranaceus, Angelica gigas, and Trichosanthes kirilowii, for preventing or treating cancer. The mixed extract of Astragalus membranaceus, Angelica gigas, and Trichosanthes kirilowii according to this embodiment of the invention does not impact normal cell growth, while having an anticancer effect which specifically inhibits the proliferation of cancer cells, and an excellent effect of inhibiting metastasis of cancer cells, and thus can be useful in preventing or treating cancer and supplementing cancer treatment.

Description

TECHNICAL FIELD[0001]The present invention relates to a composition including, as an active ingredient, a mixed extract of Astragalus membranaceus, Angelica gigas, and Trichosanthes kirilowii, for preventing or treating cancer.BACKGROUND ART[0002]Cancer is one of incurable diseases to be resolved by humanity and the huge capital has been invested in development for treating the cancer around the world. In Korea, the cancer is a first disease among causes of disease death, and more than 100,000 persons per year are diagnosed as the cancer and about more than 60,000 persons have died. A carcinogen which is the cancer initiator includes smoking, ultraviolet rays, chemicals, foods and other environmental factors, but the initiator is various and thus, it is difficult to develop medicines and effects of the medicines vary according to a generated site. Currently, since materials used as the medicines have significant toxicity and do not selectively remove only the cancer cells, it is urg...

Claims

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Application Information

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IPC IPC(8): A61K36/481A61K36/428A61K36/258A61K36/232
CPCA61K36/428A61K36/232A61K36/258A23L1/3002A23V2002/00A61K36/481A23L33/105A61P35/00A61P35/02A61K2300/00A23V2200/308A23V2250/21
Inventor KO, SEONG GYUCHO, SUNG GOOKCHOI, YOUN KYOUNGYIM, HEE SUN
Owner UNIV IND COOP GRP OF KYUNG HEE UNIV
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