Preparative separation and purification device and preparative separation and purification method

Abstract

A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path.

Description

TECHNICAL FIELD[0001]The present invention relates to a preparative separation and purification device which separates and collects one or plural components included in a solution by using a liquid chromatograph.BACKGROUND ART[0002]In a pharmaceutical field, a preparative separation and purification system using a liquid chromatograph has been used in order to store various compounds obtained by chemical synthesis as a library or collect samples for detailed analysis. As such a preparative separation and purification system, systems described in Patent Document 1 and Patent Document 2 are known.[0003]In the preparative separation and purification systems disclosed in Patent Documents 1 and 2, a target component (compound) in a sample solution is temporally separated by a liquid chromatograph to introduce the target component into a separate trap column for each target component so that the target component is trapped therein. Then, a solvent (an elution solvent) flows into each trap...

AI Technical Summary

Benefits of technology

The invention is a device that separates and purifies a target component from a solution. The device collects the target component from a trap column, which is designed to hold the target component and remove other components. The device starts collecting the target component sooner, which improves efficiency and prevents water from being included in the collected target component. The collected solvents have lower boiling points than water, which makes it possible to evaporate and dry the solvent quickly in a subsequent step.

Problems solved by technology

However, it takes several hours to about one day only by the evaporating and drying process in such a method.

However, in the liquid chromatograph, since the target component is generally introduced into the trap column together with water or a mobile phase including water as a main component to be trapped therein, a large amount of water is stored in the trap column at a time point in which the target component is completely trapped.

Even when the organic solvent is introduced into the trap column and the target component is eluted from the trap column in such a state, it is difficult to extract only the organic solvent and the target component from the trap column.

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