Processes for producing fermentation products
a technology of fermentation products and processes, applied in the field of process for producing fermentation products, can solve the problems of significant amount of residual starch material not being converted into the desired fermentation product, and achieve the effect of higher fermentation product yield
Inactive Publication Date: 2019-01-10
NOVOZYMES AS
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- Description
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- Application Information
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Benefits of technology
The use of thermostable phospholipase C during liquefaction significantly increases ethanol yield by improving the conversion of starch into fermentable sugars, outperforming conventional methods.
Problems solved by technology
Despite significant improvement of fermentation product production processes over the past decade a significant amount of residual starch material is not converted into the desired fermentation product, such as ethanol.
Method used
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[0213]Use of Thermostable Phospholipase C in Liquefaction Increases Ethanol Yield Liquefactions were performed on a 100 g scale using ground corn and backset obtained from different industrial corn ethanol plants to be used for the experiment. The dry solids (% DS) of the corn flour was 85.70% and the dry solids of the backset was 8.67%, both determined by Mettler-Toldeo HB43 halogen moisture balance. For liquefaction of the corn flour, 34.89 g corn flour was weighed into 200 mL Lab-O-Mat canisters along with 30 g backset and 35.11 g tap water to target a % DS of 32.5%. The pH of the corn slurry was adjusted to 5.0 for liquefaction.
[0214]The alpha-amylase (AA369) was used for liquefaction. In addition, thermostable Phospholipase C (PLC) from P. emersonii was evaluated at two doses, 10 and 50 μg EP / g DS. Each canister was dosed with the appropriate amount of diluted enzyme shown in Table 1 below. Alpha-amylase was dosed based on weight of ground corn and PLC was dosed based on total ...
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Abstract
The present disclosure relates to processes for producing fermentation products from starch-containing material, wherein a thermostable phospholipase C is present and / or added during liquefaction to increase fermentation product yield, such as ethanol yield. The disclosure also relates to the use of a thermostable phospholipase C in processes of the disclosure, for example, to increase fermentation product yield, such as ethanol yield.
Description
FIELD OF THE INVENTION[0001]The present disclosure relates to processes for producing fermentation products, especially ethanol, from starch-containing material. The disclosure also relates to use of a thermostable phospholipase C during liquefaction in a fermentation product production process of the disclosure to increase fermentation product yield, especially ethanol.REFERENCE TO A SEQUENCE LISTING[0002]This application contains a Sequence Listing in computer readable form. The computer readable form is incorporated herein by reference.BACKGROUND OF THE INVENTION[0003]Production of fermentation products, such as ethanol, from starch-containing material is well-known in the art. Industrially two different kinds of processes are used today. The most commonly used process, often referred to as a “conventional process”, includes liquefying gelatinized starch at high temperature using typically a bacterial alpha-amylase, followed by simultaneous saccharification and fermentation carri...
Claims
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IPC IPC(8): C12N9/16C12P7/06C12N9/28
CPCC12N9/16C12P7/06C12Y301/04011C12Y302/01001C12N9/2417C12P19/14C12Y302/01041C12Y304/21062C12Y304/23023C12Y301/03008C12Y301/04C12Y302/01003C12Y302/01004C12Y302/01008C12Y304/24039C12N9/2414C12N9/2428C12N9/2437C12N9/2457C12N9/50C12N9/20Y02E50/10Y02W30/74C12P7/64C12N9/14
Inventor KREEL, NATHANIEL EDWARD
Owner NOVOZYMES AS