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Microfluidic reaction vessel array with patterned films

a microfluidic and reaction vessel technology, applied in laboratory glassware, chemistry apparatus and processes, laboratory apparatus, etc., can solve the problem that the method of thermal cycling of materials within the reaction vessel can be slower than desired

Pending Publication Date: 2021-01-14
KRYPTOS BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a method and device for manipulating fluid samples, particularly for thermal cycling. The device includes a vacuum system to remove air from the channels and a heating system to control the temperature. The device is designed to trap cells and direct light energy to specific areas for heating, resulting in improved manipulation of cells. The technical effect is a more efficient and precise method for manipulating fluid samples for various applications.

Problems solved by technology

Unfortunately, this method of thermally cycling the materials within the reaction vessels can be slower than desired.

Method used

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  • Microfluidic reaction vessel array with patterned films
  • Microfluidic reaction vessel array with patterned films
  • Microfluidic reaction vessel array with patterned films

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Embodiment Construction

[0076]In the following detailed description, references are made to the accompanying drawings, which form a part of the description and in which are shown, by way of illustration, specific embodiments in accordance with the described embodiments. Although these embodiments are described in sufficient detail to enable one skilled in the art to practice the described embodiments, it is understood that these examples are not limiting; such that other embodiments may be used, and changes may be made without departing from the spirit and scope of the described embodiments.

[0077]Microfluidics systems or devices have widespread use in chemistry and biology. In such devices, fluids are transported, mixed, separated or otherwise processed. In many microfluidic devices, various applications rely on passive fluid control using capillary forces. In other applications, external actuation means (e.g., rotary drives) are used for the directed transport of fluids. “Active microfluidics” refers to t...

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Abstract

This disclosure describes various microfluidic devices that may be used in thermal cyclic fluid samples. Some of these devices may include a plurality of microwells that may be coupled by interconnected fluidic channels. These microwells may not be physically separated and yet may include features allowing for effective isolation of target molecules within each microwell. Other devices may include a plurality of microwells that may not be interconnected. The devices may also include mechanisms for causing a fluid to flow across the device. The devices may also include light-absorbing films for converting light energy to heat so as to allow for thermal cycling of samples within the microwells.

Description

PRIORITY[0001]This application claims benefit of U.S. Provisional Patent Application No. 62 / 872,168, filed Jul. 9, 2019, which application is hereby incorporated by reference in its entirety.BACKGROUND[0002]Reaction vessels are often used to perform various operations on DNA strands that can include operations such as polymerase chain reaction (PCR) and DNA sequencing. Polymerase chain reaction (PCR) has become an essential technique in the fields of life science, clinical laboratories, agricultural science, environmental science, and forensic science. PCR requires thermal cycling, or repeated temperature changes between two or three discrete temperatures to amplify specific nucleic acid target sequences. To achieve such thermal cycling, conventional bench-top thermal cyclers generally use a metal heating block powered by Peltier elements. Unfortunately, this method of thermally cycling the materials within the reaction vessels can be slower than desired. For these reasons, alternat...

Claims

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Application Information

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IPC IPC(8): B01L3/00B01L7/00
CPCB01L3/502715B01L2300/1861B01L2300/0816B01L7/52B01L7/525B01L2300/0883B01L2300/0877B01L7/54B01L2300/0829
Inventor SON, JUN HOBOUSSE, LUC
Owner KRYPTOS BIOTECH INC
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