Lipid emulsions and uses thereof
a technology of lipid emulsion and lipid emulsion, which is applied in the direction of anti-noxious agents, drug compositions, cardiovascular disorders, etc., can solve the problems of fat overload syndrome, volume overload of cardiovascular systems, and the decline of survival rate, so as to improve mitochondrial function and metabolism, improve tissue redox state, and improve the effect of mitochondrial function
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example 1
[0110]A lipid emulsion of the present disclosure containing 20% and 24% lipid is manufactured. Particle diameter and surface area (SA) of a prior art 20% lipid emulsion (row 1) is compared to the lipid emulsions of the example (row 2-3). The 20% lipid emulsion of the present disclosure has an increase in surface area for each droplet that is 43.5% greater than the prior art lipid emulsion.
TABLE A% lipidDiameterSA per 100 mLZeta(g / 100 mL)(nm)(×102 m2)potential20%3304.00−45mV20%2305.74−57.4mV24%2306.89Not determined
example 2
[0111]A lipid emulsion of the present disclosure containing 24% lipid was manufactured. (Of note—the same manufacturing protocol may be used to create an emulsion with 20% lipid, specifically, the amount of lipid would be decreased from 24% to 20% while the water would be analogously increased.) In this prep, sodium hydroxide 0.1 M was included at 36 ml to reach a higher pH value for the emulsion. Additionally, a homogenization pressure of about 1600 atm was used to support reduction of mean globule size, already obtained in a previous trial. Recirculation time was adjusted to guarantee at least 6 homogenization cycles. Furthermore, the N2 pressure used during homogenization was 1.0 atm.
TABLE BBATCH COMPOSITIONRaw materialAmountPercentagedescriptionper batchvaluesSojabean Oil, refined450.00g24.00%Purified Egg Phosphatide28.12g1.50%Glycerol Anhydrous35.62g1.90%NaOH 0.1M solution (~36 ml)36.0g1.60%W.F.I.1,325.26g71.00%Total1,875.00g100.00%N2 gas for blowing,1.0atmn.a.(for Homogenizati...
example 3
[0128]Experiments were performed to demonstrate the improved performance of a lipid composition of the present disclosure in a rat model of drug toxicity. The response of blood pressure in the intact anesthetized rat after 10 mg / kg bupivacaine is delivered intravenously over 10 sec followed by the infusion of 4 mL / kg of either Intralipid 20% or a composition of the present disclosure was determined. The time to recover baseline blood pressure was 3 minutes for the Intralipid composition (FIG. 1B) and only 40 sec (FIG. 1A) for a composition of the present disclosure. The responses were so different that different scales had to be used for FIG. 1A and FIG. 1B. This is evidence that a composition of the present disclosure with a greater surface area accelerates recovery from drug toxicity. Arterial pressure is an indication of the degree of toxicity caused by bupivacaine, a local anesthetic which for these purposes serves as a canonical cause of cardiac pharmacotoxicity. Thus the accel...
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