Complex of a human FOXC2 protein and a FOXC2-interacting protein

a technology of foxc2 protein and foxc2, which is applied in the field of complex of a human foxc2 protein and a foxc2interacting protein, can solve the problems that the interaction between the foxc2 protein and the groucho has not been demonstrated before, and achieve the effect of treatment or prophylaxis

Inactive Publication Date: 2007-05-15
LEANGENE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014]Yet another aspect of the invention is a method of modulating FOXC2 expression or activity, the method comprising contacting a cell expressing FOXC2, e.g., human FOXC2, with an amount of a polypeptide, e.g., a substantially pure polypeptide, described herein (e.g., a polypeptide comprising the amino acid sequence of SEQ ID NO:2, 3, 4, 5, 6, or 7), or a nucleic acid encoding the polypeptide, sufficient to modulate the expression or activity of FOXC2 in the cell.

Problems solved by technology

However, an interaction between the FOXC2 protein and Groucho has not previously been demonstrated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Identification of Putative Positive FOXC2 Interacting Clones by SRS Yeast Two-hybrid System

[0029]1.1. Overview of the Sos Recruitment System (SRS)

[0030]The Sos Recruitment System (SRS) was used to assay for polypeptides interacting with the human FOXC2 polypeptide. SRS is a modification of the well-known yeast two-hybrid system first described by Fields & Song (1989) Nature 340, 245–246.

[0031]In the CytoTrap® SRS

[0032](http: / / www.stratagene.com / vectors / signal_trans / cytotrap; see also Aronheim, A. et al. (1997) Mol. Cell. Biol. 17:3094–3102; and U.S. Pat. No. 5,776,689), proteins are expressed in the cytoplasm where, unlike in the nucleus, they may undergo posttranslational modifications. Protein-protein interactions in the cytoplasm are detected by recruitment of the human Sos gene product (hSos) to the membrane of the cell where it activates the Ras pathway. The CytoTrap system uses the unique yeast strain cdc25H, which contains a temperature-sensitive mutation in the cdc25 gene, t...

example 2

Analysis of Putative Positive Interacting Clones

[0043]2.1. Sequence Analysis of Putative FOXC2-interacting Clones

[0044]Total yeast DNA was prepared as described by Stratagene (CytoTrap XR Library Construction Kit; Instruction Manual; catalog No. 200444). The final pellet was dissolved in 20 μl H2O and used as template for PCR amplification or transformation of E. coli cells. 40 μl TOP107′ electrocompetent cells were transformed (2.5 kV, 25 μF and 200 Ω) with 2 μl of this DNA. Immediately, 1 ml of SOC medium was added and cells were incubated for 1 hour at 37° C. All cells were plated onto LB-plates containing 30 μg / ml of chloramphenicol. Transformants were used for plasmid DNA preparations (QIAGEN).

[0045]In order to amplify prey inserts the extracted yeast DNA was used as template. The PCR reaction was set up by mixing 1 μl of desired yeast DNA, 1×PCR buffer, 5 units TaqPol, 40 pmol each of the primers NA15 (SEQ ID NO:11) and NA1149 (SEQ ID NO:12) and 200 μM dNTP's to a final volume...

example 3

Characterization of FOXC2-interacting Proteins

[0053]3.1. p621

[0054]p621 (SEQ ID NO:2; partial sequence) is a protein of unknown function that interacts with the Sp1 transcription factor (Gunther, M. et al. (2000) Mol. Cell. Biol. 210: 131–142). The mouse homologue, ATFa-associated factor (mAM), has recently been cloned and characterized (De Grave, F. et al. (2000) 19: 1807–1819). It acts as a transcriptional co-repressor, and contains a bipartite NLS (Nuclear Localization Signal) and an ATPase activity.

[0055]In the present SRS screen, the interaction between FOXC2 and the p621 protein in yeast was supported by 12 obtained clones, comprising three different overlapping sequences. On basis of the identified fragments, the p621 region comprising nucleotides 580–1320 is sufficient for the FOXC2-p621 interaction.

[0056]3.2. NOLP

[0057]NOLP (for “nucleolar-localized protein”) is a nucleolar protein cloned from a human fetal brain cDNA library (Ueki, N. et al. (1998) Biochem. Biophys. Res. C...

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Abstract

The present invention relates to complexes of the FOXC2 protein with other proteins, in particular complexes of FOXC2 with proteins designated p621, NOLP, HSC71, FTP3, CLH1, and Kinase A Anchor Protein 84 / 149 (AKAP). The protein complexes can be used in methods of identifying agents useful for the treatment of medical conditions that can be treated by modulated FOXC2 activity, such as obesity, hypertriglyceridemia, diet-induced insulin resistance, and / or type 2 diabetes.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a national phase filing under 35 U.S.C. § 371 of international application number PCT / SE03 / 00139, filed Jan. 28, 2003, which claims the benefit of priority of Swedish application number 0200265-7, filed Jan. 29, 2002, and Provisional application No. 60 / 377,349, filed Apr. 30, 2002. The disclosures of the prior applications are considered part of (and are incorporated by reference in) the disclosure of this application.TECHNICAL FIELD[0002]The present invention relates to complexes of the FOXC2 protein with other proteins, in particular complexes of FOXC2 with proteins designated p621, NOLP, HSC71, FTP3, CLH1, and Kinase A Anchor Protein 84 / 149 (AKAP). The complexes can be used in methods of identifying agents useful for the treatment of medical conditions which can be treated by modulated FOXC2 activity, such as obesity, hypertriglyceridemia, diet-induced insulin resistance, and / or type 2 diabetes.BACKGROUND ART[0003]O...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A61K38/00G01N33/50A61K45/00A61P1/14A61P3/04A61P3/06A61P3/10A61P43/00C07K14/47C07K19/00C12N5/00G01N33/15
CPCC07K14/47A61K38/00A61P1/14A61P3/04A61P3/06A61P3/10A61P43/00
Inventor CLIMENT-JOHANSSON, ISABELENERBACK, SVEN
Owner LEANGENE
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