The invention discloses a single NV color center packaging method and a single NV color center device. The packaging method comprises the following steps of: a) connecting three optical fibers and setting light among the three optical fibers to transmit in a single direction, 1# optical fiber is connected with a laser and 2# optical fiber is connected with a spectrograph; b) dropping turbid liquid containing monocrystal diamond particles on the end surface of 3# optical fiber, and observing whether monocrystal diamond particles exist on the end surface of the 3# optical fiber or not, if so, entering c), and if not, repeating b); c) observing whether the typical fluorescent light of an NV color center appears on the spectrograph or not, if so, entering d), and if not, entering e); d) connecting the 2# optical fiber to a TAC (time-to-amplitude converter) to test an inverse correlation function, and judging whether the appeared NV color center is a single NV color center or not, if not, entering e); and e) washing monocrystal diamond particles away from the end surface of the 3# optical fiber, and repeating the step b). The invention simplified the single NV color center searching and positioning processes, saves the costs for searching and positioning the single NV color center and facilitates the research and application of single NV color center.