The invention relates to an anaerobic adhesive used for sealing plastic members, and a preparation method thereof. The anaerobic adhesive comprises polyurethane methacrylate, a methacrylate monomer, a plasticizer, a promoter, a promoter aid, a strengthening agent, an initiator, a polymerization inhibitor and a chelating agent. The anaerobic adhesive used for sealing plastic members has the advantages of very good sealing effect, fast positioning time, no stress damages to the plastic members, and good stability, and can be preserved at normal temperature for at least two years.