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Cymbidium plant seed asepsis sprouting and plant cultivating method

A technology of plant seeds and cultivation methods, applied in the field of aseptic germination of orchid seeds and plant cultivation, can solve problems such as no reports of aseptic germination, and achieve the effect of expanding the reproduction coefficient

Inactive Publication Date: 2008-01-02
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The germination of Cmbidium plant seeds has been studied, but the aseptic germination of the above three Cmbidium plants has not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Source of material: Agarwood tiger head orchid strains were collected from the mountainous area of ​​Yunnan. They were cultivated in the resource garden until the orchid fruit reached nine maturity and the peel was slightly yellow.

[0024] Training method:

[0025] 1. Explant treatment: sterilize the above 90% maturity fruit with 75% alcohol for 40 seconds, transfer to 1% mercuric chloride for surface disinfection for 8-12 minutes, rinse with sterile water 3-4 times, take out the seeds in the sterile room and use After wrapping the seeds with gauze, wash them with a small amount of sterile water for 5 times, then pretreat them with 0.1mol / L KOH (potassium hydroxide) solution for 8 minutes, rinse them with sterile water for 3 times, and sterilize them in the supernatant of saturated bleaching powder After 10-20 minutes, rinse with sterile water for 5 times, and then inoculate into solid medium;

[0026] 2. Culture medium: use 1 / 2MS+6-BA1.0mg.L -1 +NAA1.0mg.L -1 Cultu...

Embodiment 2

[0032] Source of material: Jasper Magnolia (C. Lowianum) orchid strains were collected from the mountainous area of ​​Yunnan, cultivated in the resource garden until the orchid fruit reaches nine maturity, and the fruit peel is slightly yellow.

[0033] Training method:

[0034] 1. The explant treatment was carried out according to the method in Example 1.

[0035] 2. Culture medium: use 1 / 2MS+6-BA2.0mg.L -1 +NAA0.5mg.L -1 Culture medium, inoculate the seeds to cultivate on this improved MS medium;

[0036] Steps 3 and 4 are carried out according to the method of Example 1.

[0037] 5. Rapid proliferation process: when the round bulb grows to about 1cm, then transfer to the proliferation medium 1 / 2MS+6-BA1.5mg.L -1 +NAA0.5mg.L -1 + Banana puree 80g / L culture;

[0038] Step 6 is carried out according to the method of Example 1.

Embodiment 3

[0040] Source of material: Cicada orchid (C. iridioides) orchid strains were collected from the mountainous area of ​​Yunnan, cultivated in the resource garden until the orchid fruit reaches nine maturity, and the fruit peel is slightly yellow.

[0041] The cultivation method is carried out according to the method of Example 1 or Example 2.

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PUM

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Abstract

The invention discloses a method of sterile germination of seed and plant cultivating for thoroughwort. Collecting Cmbidium tracyanum L castle plants, C. iridioides plants and jasper orchid plants and cultivating them in the resource garden until the fruit of the orchid is 90% mature. The cultivating method compeises: culturing seeds in the modified culture medium by explant, trans-culturing when the seed grows sprout and root with root hair, rapid breeding and root-growing culturing, new root will grow out one month later. The traditional orchid breeding is primarily the single-plant propagation with a low propagation rate, the portion between old sprout and mew sprout is only about 1:1-3,and is hard for mass production. The invention applies modern biotechnology and breed millions of orchids only employing one sprout in one year, which can meet the orchid industrialization demand; sterilizing technique can also be proceeded and produce seeds free of virus, which deduces the production cost, increase the product quality and meet the orchid industrialization demand effectively.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for aseptic germination and plant cultivation of orchid seeds. Background technique [0002] Cmbidium tracyanum L castle, C. Lowianum and C. iridioides are plants of the Orchidaceae family, with large flowers and long branches and high ornamental value. They are excellent orchid resources. The three orchids are mostly produced in Yunnan, Guizhou and southeastern Tibet, and are good hybrid parents and cut flower materials. [0003] Orchid seeds are small and contain some incompletely developed spherical embryos without endosperm. It is difficult to germinate in a natural state. It needs to be symbiotic with orchid bacteria or cultured under sterile conditions to succeed. The germination of Cmbidium plant seeds has been studied, but the aseptic germination of the above three Cmbidium plants has not been reported. Contents of the invention [0004] The object ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00
Inventor 李枝林余朝秀王卜琼王玉英黄丽萍
Owner YUNNAN AGRICULTURAL UNIVERSITY