Microbial fermenting preparation process for rice separated protein

A technology of microbial fermentation and protein separation, applied in plant protein processing, etc., can solve the problems of food safety hazards, large environmental impact, damage to the kidneys, etc., and achieve the effects of reduced production costs, less environmental pollution, and food safety

Inactive Publication Date: 2008-07-09
桃源县万福生科粮油加工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the commonly used methods for extracting rice protein are "alkali method" or "high temperature amylase liquefaction method". These methods have three fatal weaknesses: first, the extracted protein is severely denatured and has low activity, especially for the first limiting amino acid lysine Acid is very destructive, and after eating, the digestible absorption utilization rate of the human body is significantly reduced; the second is that after high-concentration lye treatment, it may also cause unexpected consequences and potential toxicity, and there are hidden dangers in food safety, such as the production of lysyl alanine Acid (Lysinoalanine), this substance will damage the kidneys, and may also cause non-protein components to co-precipitate with proteins to reduce the quality of separation; the third is serious environmental pollution. After the rice protein isolate is prepared with lye, there are certain difficulties in the treatment of waste liquid , has a greater impact on the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Weigh 5 kilograms of rice, break it into 50 mesh fineness, add 5 times of drinking tap water, soak for 5 hours; (2) add 0.5% sucrose, 0.1% peptone, 1% soybean powder, 0.2% corn extract, 0.1% potassium dihydrogen phosphate, mix evenly, heat to 80°C, and keep warm for half an hour to form a gelatinized rice slurry; (3) when the rice slurry is cooled to 37°C, inoculate the bacillus ( B.amyloliqefaciens), the expansion culture medium of bacillus is 2% sucrose, 0.5% peptone, 10% soybean powder, 0.5% corn extract, 0.5% potassium dihydrogen phosphate, expands and cultivates by the method for routine microbial expansion; The rice slurry inoculated with Bacillus was stirred and cultured at 37°C for 48 hours, then heated to 45°C and kept for 5 hours, and cooled to 25°C; (4) Filter the liquefied liquid with a plate basket to remove the coarse residue, and the filtrate was concentrated by membrane classification filtration , and then vacuum-dry the concentrate at low temperatur...

Embodiment 2

[0015] Weigh 5 kg of rice, break it into 80 mesh fineness, add 5 times of drinking tap water and soak for 6 hours; add 0.5% sucrose, 0.1% beef extract, 1% soybean powder, 0.1% potassium dihydrogen phosphate, mix well, Heat to 90 DEG C and keep warm for 20 minutes to form a gelatinized rice slurry; when the rice slurry is cooled to 37 DEG C, inoculate and expand the cultured Bacillus subtilis that produces liquefied amylase, and the expansion medium of the Bacillus is 2% sucrose, 0.5 % peptone, 10% soybean powder, 0.5% corn extract, 0.5% potassium dihydrogen phosphate, expand the cultivation by the method of conventional microbial expansion cultivation, and inoculate the rice slurry of bacillus at 35 ℃ for 55 hours, then heat up to Keep at 50°C for 4 hours; cool to 25°C, filter with a plate basket, remove the coarse residue, and concentrate the filtrate through membrane grade filtration, dry the concentrate at low temperature and vacuum into powder, and obtain high-activity rice...

Embodiment 3

[0018] Weigh 10 kg of rice, break it into 90 mesh fineness, add 5 times of drinking tap water and soak for 6 hours; add 0.5% sucrose, 0.1% beef extract, 1% soybean powder, 0.1% peptone, 0.1% potassium dihydrogen phosphate , mix evenly, heat at 85°C, and keep warm for 30 minutes to form a gelatinized rice slurry; when the rice slurry is cooled to 40°C, inoculate the bacillus (Bacillus subtilis) that has been expanded and cultivated to produce liquefied amylase, and the expansion medium of the bacillus is 2% sucrose, 0.5% peptone, 10% soybean powder, 0.5% corn extract, 0.5% potassium dihydrogen phosphate, expand the culture according to the method of conventional microbial expansion culture, and inoculate the rice slurry of Bacillus at 40 ° C for 50 Then heat up to 48°C and keep for 5 hours; cool to 20°C, filter the plate basket to remove the coarse residue, filter the filtrate through membrane classification and concentrate, and spray-dry the concentrated solution into powder to...

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Abstract

A process for preparing rice protein by microbial fermenting method includes such steps as crushing rice grains, immersing, adding at least one of cane sugar, soybean powder, potassium dihydrogen phosphate, beef extract, peptone, liquid extract of corn and yeast extract, heating at 80-100 deg.C for 20-60 min, cooling to 20-40 deg.C, inoculating bacillus, fermenting for 2-3 days, liquefying for 5-10 hrs, filtering, concentrating and drying.

Description

technical field [0001] The invention relates to a preparation method of rice protein isolate, in particular to a kind of high biological value (BV) and protein efficacy ratio (PER), which can be widely used in children's food, special dietary food for middle-aged and elderly people, or functional health food. A microbial fermentation preparation method for rice protein isolate. Background technique [0002] Rice protein is a high-quality grain protein: first, it has high biological value (BV) and protein efficacy ratio (PER), which is close to that of fish and shrimp; second, it is hypoallergenic, making it very suitable as a nutritional supplement for infants or special populations In food, the application range is very wide. [0003] At present, the commonly used methods for extracting rice protein are "alkali method" or "high temperature amylase liquefaction method". These methods have three fatal weaknesses: first, the extracted protein is severely denatured and has low...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/14
Inventor 林亲录
Owner 桃源县万福生科粮油加工有限公司
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