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Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character

A hybrid wheat and blue-grain technology, which is applied in the field of two-line hybrid wheat breeding, can solve the problems of high risk, high cost of chemical andricides, and reduced pistil activity, so as to avoid the influence of bad genes and enhance heterosis , The effect of simple breeding method

Inactive Publication Date: 2008-09-24
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Three-line CMS breeding is always 3-5 years behind conventional breeding, and it is difficult to surpass conventional breeding; twice outcrossing, high risk and high seed production cost (Sang Wei, Han Xinnian, etc., 2005; Liu Xunsheng, 2005)
However, there are also defects. For example, the effect of chemical killing is closely related to the dosage, processing time and the growth and development period of wheat. If the medication tool is not precise enough, improper medication will cause incomplete androcidal or reduced pistil activity, thereby affecting the purity and quality of hybrids. Seed Production
The cost of existing chemical andricides is relatively high, and the cost of using the agent for hybrids is about 0.70 yuan / kg, and the effect is not very satisfactory, which still affects the utilization of hybrid wheat

Method used

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  • Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character
  • Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character
  • Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character

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Experimental program
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Effect test

specific Embodiment approach

[0035] Establishment of ms-Rht3 gene linkage:

[0036] For 94.18.26 [(the genotype is ms-rht3 / Ms-Rht3, in which the ms sterility gene, namely ms1b, comes from the "Probus" mutant, and the Rht3 dwarf gene comes from Nainari Rht3; the 4B apical deletion derived from the "Probus" mutant Genetic Research on Chromosomes, "Northwest Agricultural Journal", Vol. 8, No. 4, 1999 (pp. 25-29)] 116 individual plants of the self-inbred progeny were potted in the greenhouse. Since the Rht3 gene was insensitive to gibberellic acid, it was The seedling stage was treated with 10 ppm gibberellic acid (GA3), and then the seedlings were classified by height and dwarf and transplanted to the field, and the survey results of plant height after heading were consistent with the classification at the seedling stage. The fertility rate was 93.1%. Among the 60 semi-dwarf (60-65cm) groups, the sterile rate was 1.7%, that is, 2 plants were sterile. Among the 27 dwarf (40-45cm) groups, the sterile rate was ...

Embodiment 1

[0043] 1) Embodiment 1 (method one):

[0044] The original dual-purpose line T0010-5B-1LB-1 and the elite line 949-14 were used to prepare a hybrid combination, the combination number is x02163, in F 1 Substitute only blue seeds for planting, from F 2 The light blue seeds are sorted out from the replacement seeds for planting, and the excellent individual plants are selected. will F 3 The white-grained and light-blue-grained seeds in each line were planted separately, each with about 50 plants, and 25% or 100% of the white-grained population were selected and harvested. And so on, until the line is stable and the isolated white grain population is sterile. X02163-8-1-8, X02163-8-1-12, X02163-8-7-1, X02163-8-7-2, X02163-8-7-4, X02163-8-7- have been selected 10 and other new light blue grain dual-purpose lines. These dual-purpose lines are weak winter, strong tillering ability, moderate resistance to stripe rust and scab, plant height 60-75cm, medium-early maturity, mature ...

Embodiment 2

[0045] 2) Embodiment 2 (method two):

[0046] Select the F of the cross between the sterile line X9701-1-1 and the excellent line JYP97-1-1 2 The excellent sterile plant isolated from the generation is a cross between the female parent and the dual-purpose line T0010-5B-1LB-1, and the combination number is X01098, which is the same as the first method. 1 Substitute light blue seeds, F 2 Substitute light blue seeds for planting, select excellent individual plants, F3 The excellent lines are screened above, but the difference is that only light blue seeds are planted in the remaining generations until the lines are stable and the white-grain sterile line needs to be isolated. X01098-1-2-4-3-2, X01098-1-2-4-3-3, X01098-1-2-4-4-1, X01098-1-2-4-4- have been selected 2. Several new dual-purpose systems such as X01098-1-2-4-4-11, X01098-1-2-4-4-13, X01098-1-2-4-4-15, etc. These dual-purpose lines are weak spring, strong tillering ability, moderate resistance to stripe rust and sca...

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Abstract

The invention discloses a breeding method of bilinear wheat hybrid taking blue granule as mark property. The method firstly establishes original bilinear system, then establishes breeding method for new bilinear, and at last prepares hybrid combination to sift dominant combination for production. The invention provides a new way for using wheat hybrid advantage, reduces effectively the cost for hybrid breeding and producing and possesses wide application prospect. The breeding of sterile line will not be lagged behind from that of normal breed when producing hybrid by this invention; the fruit got from bilinear auto-copulation is normal and the breeding coefficient of sterile is large; the fruit of hybrid of sterile line with arbitrary good wheat strain is normal; the good cytoplasm of out source can be replanted into sterile line to improve hybrid advantage; there is no bad influence in the sterile gene and 4thS.4AgL additive displacement colorant layer.

Description

technical field [0001] The invention relates to the fields of cytogenetics, chromosome engineering, biochemistry, wheat genetics and breeding, utilization of recessive nuclear sterility genes in wheat heterosis and the like, in particular to a two-line hybrid wheat with blue grain as a marker trait. Breeding method. Background technique [0002] Wheat is one of the main food crops in my country. With the reduction of arable land and the increase of population, it is imperative to increase the yield per unit of wheat. The utilization of heterosis is an important way to improve the yield of wheat, and the hybridization of wheat varieties is the need and inevitable trend of the development of the seed industry. Crops such as rice, corn, and sorghum have long been hybridized, and have been widely used in production successively, greatly increasing the yield, making the breakthrough in wheat breeding to be pinned on the utilization of heterosis, and constantly exploring new ways...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 李中安
Owner NORTHWEST A & F UNIV