Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character
A hybrid wheat and blue-grain technology, which is applied in the field of two-line hybrid wheat breeding, can solve the problems of high risk, high cost of chemical andricides, and reduced pistil activity, so as to avoid the influence of bad genes and enhance heterosis , The effect of simple breeding method
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specific Embodiment approach
[0035] Establishment of ms-Rht3 gene linkage:
[0036] For 94.18.26 [(the genotype is ms-rht3 / Ms-Rht3, in which the ms sterility gene, namely ms1b, comes from the "Probus" mutant, and the Rht3 dwarf gene comes from Nainari Rht3; the 4B apical deletion derived from the "Probus" mutant Genetic Research on Chromosomes, "Northwest Agricultural Journal", Vol. 8, No. 4, 1999 (pp. 25-29)] 116 individual plants of the self-inbred progeny were potted in the greenhouse. Since the Rht3 gene was insensitive to gibberellic acid, it was The seedling stage was treated with 10 ppm gibberellic acid (GA3), and then the seedlings were classified by height and dwarf and transplanted to the field, and the survey results of plant height after heading were consistent with the classification at the seedling stage. The fertility rate was 93.1%. Among the 60 semi-dwarf (60-65cm) groups, the sterile rate was 1.7%, that is, 2 plants were sterile. Among the 27 dwarf (40-45cm) groups, the sterile rate was ...
Embodiment 1
[0043] 1) Embodiment 1 (method one):
[0044] The original dual-purpose line T0010-5B-1LB-1 and the elite line 949-14 were used to prepare a hybrid combination, the combination number is x02163, in F 1 Substitute only blue seeds for planting, from F 2 The light blue seeds are sorted out from the replacement seeds for planting, and the excellent individual plants are selected. will F 3 The white-grained and light-blue-grained seeds in each line were planted separately, each with about 50 plants, and 25% or 100% of the white-grained population were selected and harvested. And so on, until the line is stable and the isolated white grain population is sterile. X02163-8-1-8, X02163-8-1-12, X02163-8-7-1, X02163-8-7-2, X02163-8-7-4, X02163-8-7- have been selected 10 and other new light blue grain dual-purpose lines. These dual-purpose lines are weak winter, strong tillering ability, moderate resistance to stripe rust and scab, plant height 60-75cm, medium-early maturity, mature ...
Embodiment 2
[0045] 2) Embodiment 2 (method two):
[0046] Select the F of the cross between the sterile line X9701-1-1 and the excellent line JYP97-1-1 2 The excellent sterile plant isolated from the generation is a cross between the female parent and the dual-purpose line T0010-5B-1LB-1, and the combination number is X01098, which is the same as the first method. 1 Substitute light blue seeds, F 2 Substitute light blue seeds for planting, select excellent individual plants, F3 The excellent lines are screened above, but the difference is that only light blue seeds are planted in the remaining generations until the lines are stable and the white-grain sterile line needs to be isolated. X01098-1-2-4-3-2, X01098-1-2-4-3-3, X01098-1-2-4-4-1, X01098-1-2-4-4- have been selected 2. Several new dual-purpose systems such as X01098-1-2-4-4-11, X01098-1-2-4-4-13, X01098-1-2-4-4-15, etc. These dual-purpose lines are weak spring, strong tillering ability, moderate resistance to stripe rust and sca...
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