Coated column for capillary electrophoresis
A technology of capillary electrophoresis and capillary, which is applied in the direction of material analysis, measuring devices, and instruments through electromagnetic means, which can solve the problems of insufficient coating thickness, low coating concentration, and inability to well suppress silicon hydroxyl ionization, and achieve chain-free Winding solubility, the effect of good solubility
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example 1
[0059] Blank column: at pH=5, the separation spectrum of three basic proteins, cytochrome C, lysozyme, and α-chymotrypsinogen A, is shown in Figure 2 a, which cannot be separated; at pH=4.5, the separation of adenine, adenosine and 6-furfuryl aminopurine three bases and their derivatives such as Figure 2b , the separation effect is not good, and the column efficiency is generally 1.8×10 4 , The separation time is too long, and it takes nearly 50 minutes to separate completely.
example 2
[0061] With tetrachlorosilane as the central starting core, allylmagnesium bromide and methylhydrogendichlorosilane as growth monomers, the Grignard reaction and hydrosilylation reaction were repeated three times to obtain 3 generations of organic compounds terminated by silicon-chlorine bonds. Silicon dendrimers.
[0062] 3rd generation silicone dendritic macromolecule physical adsorption coating column capped with silicon-chloride bonds: Prepare the coated capillary electrophoresis column as described in 2.1.1, and test its column performance. Get the spectrum as Figure 2c , the average separation column efficiency reaches 9×10 4 , Within 20 minutes, the three bases of adenine, adenosine and 6-furfurylaminopurine and their derivatives can be completely separated.
example 3
[0064] With tetramethyltetravinylcyclotetrasiloxane as the center starting core, methylhydrogendichlorosilane and allylmagnesium bromide as growth monomers, repeated hydrosilylation, Grignard reaction 3.5 times to get 3.5 Organosilicon dendrimers terminated with silicon-chloride bonds
[0065] 3.5-generation silicone dendrimer chemically bonded coating column capped with silicon-chlorine bonds (KH-550 is the coupling agent): Prepare a coated capillary electrophoresis column as described in 2.2.1, and test its column performance. Get the spectrum as Figure 2d , the average separation column efficiency reaches 12×10 4 , The three substances can be completely separated within 18 minutes.
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