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A method of culturing osmunda japonica tissues

A technology of tissue culture and Osmanthus japonica, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve problems such as seasonal cutting restrictions, and achieve the effect of breaking through seasonal restrictions

Inactive Publication Date: 2009-03-04
HUBEI HUAYI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the tissue culture of Chinese cabbage adopts aseptic germination of spores, and some spores are directly sown in the greenhouse matrix to germinate into seedlings, but the tissue culture through spore germination is limited by seasonal cuts.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The steps of the tissue culture of Osmanthus fruticosa are as follows:

[0049] 1. Remove all the expanded leaves and roots of the Osmanthus cabbage, leaving only the young stem tip, and rinse it with tap water. Then disinfect it with 0.1% mercury liter for 5-20 minutes on the ultra-clean workbench, and finally rinse it with sterile water for later use.

[0050] 2. Insert the sterilized stem tips of Osmanthus chinensis into the induction medium, culture in the dark at 20°C-27°C for 3-7 days, and then light culture, the intensity gradually increases from weak to strong. A large number of protocorms can be formed on young leaves in about 20-40 days.

[0051] Based on the dosage of one liter of medium, the formulation of induction medium is:

[0052] MS 250ml

[0053] Kinetin 0.5 mg

[0054] Sodium dihydrogen phosphate 100 mg

[0055] Activated Charcoal 1000mg

[0056] Sucrose 30000 mg

[0057] Agar 9000 mg

[0058] 3. Protocorms continue to be cultivated for 30 da...

Embodiment 2

[0076] The steps and culture conditions of Weicai tissue culture are the same as in Example 1, and the induction medium formula is (with the consumption of one liter of medium)

[0077] MS 2500ml

[0078] Kinetin 2mg

[0079] 3-Indolebutyric acid 2mg

[0080] Sodium dihydrogen phosphate 1000mg

[0081] Activated Charcoal 2000mg

[0082] Sucrose 30000mg

[0083] Agar 9000mg

[0084] The subculture medium formula is (according to the amount of one liter of medium)

[0085] MS 2500ml

[0086] Kinetin 2mg

[0087] 3-Indolebutyric acid 2mg

[0088] Sodium dihydrogen phosphate 1000mg

[0089] Activated Charcoal 2000mg

[0090] Sucrose 30000mg

[0091] Agar 9000mg

[0092] The formula of rooting medium is (in the amount of one liter of medium)

[0093] MS 2500ml

[0094] Kinetin 2mg

[0095] Sodium dihydrogen phosphate 1000mg

[0096] Activated Charcoal 2000mg

[0097] Sucrose 30000mg

[0098] Agar 9000mg

Embodiment 3

[0100] The steps and culture conditions of Weicai tissue culture are the same as in Example 1, and the induction medium formula is (with the consumption of one liter of medium)

[0101] MS 4000ml

[0102] Kinetin 4 mg

[0103] 3-indolebutyric acid 4 mg

[0104] Sodium dihydrogen phosphate 2000mg

[0105] Activated Charcoal 3000 mg

[0106] Sucrose 30000mg

[0107] Agar 9000mg

[0108] The subculture medium formula is (according to the amount of one liter of medium)

[0109] MS 4000ml

[0110] Kinetin 4 mg

[0111] 3-indolebutyric acid 4mg

[0112] Sodium dihydrogen phosphate 2000mg

[0113] Activated Charcoal 3000 mg

[0114] Sucrose 30000 mg

[0115] Agar 9000mg

[0116] The formula of rooting medium is (in the amount of one liter of medium)

[0117] MS 4000ml

[0118] Kinetin 4 mg

[0119] Sodium dihydrogen phosphate 2000 mg

[0120] Activated Charcoal 3000 mg

[0121] Sucrose 30000 mg

[0122] Agar 9000mg

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Abstract

A tissue culture method for common vetch herb includes such steps as providing the stem tip of common vetch herb as explant, culturing for inducing protocorm, reproduction culture, rooting culture, training seedlings and transplanting.

Description

technical field [0001] The invention relates to a method for fern tissue culture, in particular to a method for tissue culture of vetch. Background technique [0002] Weicai is a kind of pure natural, pollution-free green health food that grows in the mountains and forests. It is rich in 17 kinds of nutrients needed by the human body, such as protein, iron, calcium, selenium, etc., and has a unique crisp and refreshing feature. It is loved by customers from Japan, South Korea, Southeast Asia and other countries, and is known as "Lin Haishan Treasure". Due to the slow propagation of conventional ramets, we all adopt the method of tissue culture for propagation. Han Jing and Zhao Li pointed out in their article "Research Progress on Fern Reproduction" (Anhui Agricultural Science 2005 Vol.33No.7 P.1261-1263) that the tissue culture of various ferns is mainly the aseptic germination of spores, that is, the spores After the surface is sterilized, spread it evenly on the medium,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 徐年声艾德甫
Owner HUBEI HUAYI BIOTECH CO LTD
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