Composition for preventing atherosclerosis
A composition and drug technology, applied in the direction of drug combination, food science, plant raw materials, etc., can solve the problem of arteriosclerosis with unclear antioxidant activity, and achieve the effect of no side effects and low toxicity
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Embodiment 1
[0037] Embodiment 1 (in vitro antioxidant data)
[0038] Add 500ml of 90% capacity ethanol water to 100g of safflower meal after oil extraction, heat and stir in a hot water bath at 60°C for 3 hours, and then filter. The same operation was performed once on the filtered solid portion, and the obtained filtrates were combined and concentrated under reduced pressure to obtain 60 ml of a concentrate. Add water to the concentrated solution to make 200ml quantitatively, suspend the content, then wash twice with 120ml of n-hexane, extract the washed water layer with 100ml ethyl acetate, and extract twice in total. The ethyl acetate extract was washed with saturated brine, and the ethyl acetate layer was dehydrated with anhydrous magnesium sulfate, filtered, and concentrated under reduced pressure to obtain 1.16 g of a solid. At the same time, the rapeseed meal, soybean meal, soybean germ meal, and soybean seed coat after oil extraction were also treated in the same way, and 1 ml of...
Embodiment 2
[0041] Example 2 (arteriosclerosis prevention effect in vivo)
[0042] Extracts of rapeseed meal and safflower meal were prepared as follows.
[0043] Add 3000ml of 90% capacity ethanol water to 600g of oil-pressed rapeseed meal, heat and stir in a hot water bath at 60°C for 3 hours, and then filter. The same operation was performed once on the filtered solid portion, and the obtained filtrates were combined and concentrated under reduced pressure to obtain 500 ml of a concentrate. Add water to the concentrated solution, quantitatively 1000ml, suspend the content, then wash twice with 500ml of n-hexane, extract the washed water layer with 500ml ethyl acetate, and extract twice in total. The ethyl acetate layer was dehydrated with anhydrous magnesium sulfate, filtered, and then concentrated under reduced pressure to obtain 12.5 g of a solid substance.
[0044] The same operation as above was carried out on 600 g of safflower meal after oil expression to obtain 10.1 g of extra...
Embodiment 3
[0050] Male apoE knockout mice (purchased from Jackson Laboratory) aged 6-7 weeks were divided into control / 0.2% by weight serotonin derivative administration groups (p-coumaroyl 5-serotonin (CS), feruloyl 5-serotonin (FS) 0.1% each) (CS+FS, 0.2%) / 0.4% by weight serotonin derivative administration group (p-coumaroyl 5-serotonin (CS), feruloyl 5-serotonin (FS) each 0.2% ) (CS+FS, 0.4%) / 0.4 wt% feruloyl serotonin (FS, 0.4%) / 1 wt% safflower meal extract (SFM) administration group (SFM, 1%), a total of 5 groups, There were 7-10 birds in each group, and each group was allowed to freely ingest the baits shown in Table 2 for 15 weeks. The extract (SFM) of safflower meal used in this example was prepared according to the method shown in Example 2. After the end of the administration period, the mice were sacrificed, and sections of the aortic root were prepared, and the lipid deposition sites (arteriosclerotic lesions) were stained with Oil Red 0. Three slices were prepared for each...
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