Biochip hybridizing box
A technology of biochip and hybridization box, which is applied in the direction of biological testing, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of insufficient versatility, limitation of the application range of hybridization box, and influence on the application of biochips, so as to prevent Volatile effect
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Embodiment 1
[0037] Chip Hybridization Kit
[0038]The size of the two clamping grooves in the chip hybridization box is the same, the length of each clamping groove is 104mm, the width is 11.1mm, the opening distance is 12.9mm, and the wall thickness is 2.1mm. The height of the bottom plate of the upper cover is 2.6mm, the height of the raised groove is 4mm, and the wall thickness is 2.6mm. The height of the bottom plate of the base is 2.6mm, and the height of the surrounding groove around the bottom plate is 7.4mm; the length of the internal chip reaction area is 75.9mm, and the width is 26mm; the outer diameter of the positioning column defining the bracket in the reaction area is 3.9mm. The width of the bracket is 25.9mm, the length is 81.5mm, there is a protruding block about 75.5mm from the top, which is used to cooperate with the positioning post to fix the bracket; there is a gap at the top of the bracket. The material of the O-ring on the base is silicon rubber, and the cross-sec...
Embodiment 2
[0040] The reaction of ordinary chips in the hybridization box
[0041] See Figure 9 and 10 Place the bracket 4 on the reaction area of the base 1 of the hybridization box, place the chip 17 with the dot matrix facing up on the upper surface of the bracket 4, and add 0.5ml of purified water to the base 1 The reaction area can also be added to the water-absorbing material 16 on the bracket 4. Add 100 μL of prepared hybridization solution to the position of the array on the chip, and place the cover slip 18 (LifterSlips TM ) is stamped on the surface of the chip 17. Then, the upper cover 2 of the hybridization box was buckled into the base 3, and two clip slots 3 were stuck on, and put into a water bath at 42°C for a hybridization reaction for 12 hours. After the hybridization reaction, take the hybridization box out of the water bath, take out the clamping tank, then take out the chip 17 from the bracket, remove the cover slip 18, and put the chip 17 in 0.1% SDS cleaning...
Embodiment 3
[0043] The reaction of the fence chip in the chip hybridization box
[0044] See Figure 11 and 12 , put the bracket 4 in the reaction area of the base of the hybridization box, and fix the sample on the 12-well SmartGrid containing the gene sample TM The fence chip 19 is placed on the upper surface of the carriage 4, wherein the face containing the fence (that is, with a dot matrix) is facing up, and the SmartCover TM The cover slip 20 is placed on the upper surface of the fence chip; then 1 ml of purified water is added to the chip reaction area of base 2 of the hybridization box; 30 μL of reaction solution is added to each fence. Buckle the upper cover into the base, snap it into the clip slot, and put it in an oven at 37°C for 2 hours to react. After the reaction, take the hybridization box out of the oven, remove the clip slot from the hybridization box, and then take out the fence chip from the bracket, remove the cover piece, wash the fence chip in 0.1% PBST clea...
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