Unlock instant, AI-driven research and patent intelligence for your innovation.

Pesticide residue enzymatic detection method using same kind deactivated enzyme as reference and its device

A technology for pesticide residues and detection methods, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of high detection cost, time-consuming and laborious, unknown methods and devices, etc., and achieve rapid detection and low detection cost. , The effect of simple detection steps

Inactive Publication Date: 2009-06-17
UNIV OF SHANGHAI FOR SCI & TECH
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional detection method has the disadvantages of complicated test steps, time-consuming and laborious, professional operation, and high detection cost, so it cannot be used for on-site detection and rapid detection.
In recent years, although relevant papers have been published internationally, there are no methods and devices available for implementation.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pesticide residue enzymatic detection method using same kind deactivated enzyme as reference and its device
  • Pesticide residue enzymatic detection method using same kind deactivated enzyme as reference and its device
  • Pesticide residue enzymatic detection method using same kind deactivated enzyme as reference and its device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] The device that this method adopts is shown in Fig. 1, 2, comprises light source 1, is made up of reaction chamber 2, reference chamber 3, storage tank 13,14,15 constant temperature cuvette frame 16, fan 4, photosensitive tube 5 and The signal processing device is composed of amplifier 6, A / D converter 7, single-chip microcomputer 8, printer 9, display 10, keyboard 11, and graded sound and light alarm 12. The signal processing device can also be a graded sound and light alarm composed of a differential amplifier 17 , a comparator 18 and a sound and light generator composed of a sound generator 20 and a light emitting diode 21 . The light source 1 is composed of light-emitting diodes to form a double light path, and the two paths of light with the same wavelength pass through the reaction chamber 2 and the reference chamber 3 at the same time. Depend on image 3 As shown, a miniature magnetic stirrer 22 is placed below the reaction chamber 2 / reference chamber 3, and a s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A process for detecting the residual agricultural chemicals by using the deactivated same enzyme as reference includes making the color comparison utensil holder in constant-temp state, loading the specimen respectively in reaction chambers and reference chambers, which contain respectively the active enzyme, deactive enzyme, substrate and coloring liquid, stirring, light irradiation, receiving optical signals, and signal processing to detecte the residual agricultural chemicals. Its equipment is composed of light source, constant-temp color comparison utensil holder including reaction chambers, reference chamber and storage, photosensing tube and signal processing device. Its advantages are low cost and fast detection.

Description

technical field [0001] The invention relates to an enzymatic detection method and device for pesticide residues using the same inactivated enzyme as a reference. Background technique [0002] Organophosphorus pesticides are currently commonly used pesticides. Organophosphorus pesticides have small accumulation in animals, and have the characteristics of fast degradation and low residues. However, organophosphorus pesticides have high residues in plant foods, especially fruits and vegetables. The absorption of organic phosphorus has strong acute toxicity, and it is generally stipulated that the application should be prohibited within a few days before harvest. However, unscrupulous fruit farmers and vegetable farmers spray a large amount of pesticides before the sale of vegetables and fruits, which is the main cause of acute toxicity when consumers eat them. . To prevent agricultural products with excessive pesticide residues from going on the market, one of the important me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/25
Inventor 肖建军徐斐许学勤华澤钊赵肖磊
Owner UNIV OF SHANGHAI FOR SCI & TECH