Preparation of cotton-seed polypeptide with anti-oxidant function
A technology of anti-oxidation and production methods, which is applied in the field of nutritious food and functional, can solve the problems of different characteristics of hydrolyzate, loss of activity of hydrolyzate, time consumption of energy and other problems, and achieve shortening of hydrolysis time, wide application and pH value simple effect
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Embodiment 1
[0015] Embodiment 1, the production of the dry powder of cottonseed polypeptide with antioxidant effect
[0016] Take 20 kg of cottonseed protein isolate with a protein content of 60%, add 60 kg of distilled water, grind it into a fine and uniform cottonseed protein isolate slurry with a colloid mill, add 320 kg of distilled water, put it into the reaction tank, and use 1mol / L Sodium hydroxide solution to adjust its pH value to 7.9, add 1 kg of alkaline protease labeled Alcalase (in the state of continuous stirring, and heat to 54 ° C for hydrolysis, constantly use 1mol / L sodium hydroxide solution in the hydrolysis process Adjust and maintain the pH value of 8.0, keep the temperature at 54°C, and continue the hydrolysis for 1 hour. After the first hydrolysis, adjust its pH value to 7.0 with a molar concentration of 1mol / L hydrochloric acid solution, and adjust the temperature to 50°C. In the state of constant stirring, add the label Flavourzyme TM 1.2kg of flavor protease, in...
Embodiment 2
[0017] Embodiment 2, the production of the dry powder of cottonseed polypeptide with antioxidant effect
[0018] Take 18 kg of cottonseed protein isolate with a protein content of 70%, add 72 kg of distilled water, grind it into a fine and uniform cottonseed protein isolate slurry with a colloid mill, then add 280 kg of distilled water, put it into the reaction tank, and use 1mol / L Sodium hydroxide solution to adjust its pH value to 8.0, add 0.8kg of alkaline protease labeled Alcalase under continuous stirring, and heat to 55°C for hydrolysis, and continuously use 1mol / L sodium hydroxide solution during the hydrolysis process Adjust and maintain the pH value at 7.9, maintain the temperature at 55° C., and continue the hydrolysis for 1.5 hours. After the first hydrolysis, adjust the pH value to 7.1 with a hydrochloric acid solution with a molar concentration of 1mol / L, adjust the temperature to 49°C, and add Flavourzyme under constant stirring. TM 0.8kg of flavor protease, dur...
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