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Compound injection of isatis root and motherwort

A technology of motherwort and radix isatidis is applied in pharmaceutical formulations, medical preparations containing active ingredients, and medical raw materials derived from angiosperm subphylum. , Inhibition of platelet aggregation in vitro and in vivo

Inactive Publication Date: 2009-07-29
于荣敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As we all know, the main components of promoting blood circulation and removing blood stasis in Motherwort are alkaloids, such as Leonurine, Stachydrine, Leonurine A and Leonurine B, etc., so only a small number of active substances in Motherwort can be extracted by the above extraction method. Element

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take the total weight of 2 kg of Radix Radix and Motherwort in this formula with a weight ratio of 1:3, add 4 times the amount of 80% alcohol aqueous solution, heat and reflux for extraction twice, each time for 2 hours, combine the extracts, filter, and add 6 times the amount of the filtrate (Volume ratio) 95% alcohol aqueous solution, let it stand overnight, centrifuge for 10 minutes (20000g), concentrate the supernatant under reduced pressure again, add 2% activated carbon and boil for 20 minutes, filter. The filtrate was adsorbed on the AmberliteXAD2 adsorption resin column, and then eluted with lower alcohols. Concentrate the eluate, perform ultrafiltration with a 0.4μm filter membrane, add sterile water for injection to a volume of 1000ml, and distribute it in 5ml ampoules to make small needles for intramuscular injection.

Embodiment 2

[0023] Take 5kg of the total weight of Radix Isatidis and Motherwort in this formula with a weight ratio of 1:5, add 6 times the amount of 55% alcohol aqueous solution, heat and reflux extract twice, each time for 2 hours, combine the extracts, filter, add 6 times the amount of filtrate (Volume ratio) 95% alcohol aqueous solution, let it stand overnight, centrifuge for 10 minutes (20000g), concentrate the supernatant under reduced pressure again, add 2% activated carbon and boil for 20 minutes, filter. The filtrate was adsorbed on the AmberliteXAD2 adsorption resin column, and then eluted with lower alcohols. Concentrate the eluate, perform ultrafiltration with a 0.4 μm filter membrane, and add sterile water for injection to a volume of 1000 ml. Measure the pH value and content, filter until clear after passing the test, fill and seal, and sterilize by autoclaving at 115°C for 30 minutes, then it can be made into a large infusion solution for intravenous injection.

Embodiment 3

[0025] Take the total weight of Radix Radix Radix and Leonurus 3kg in the weight ratio of 1:6.5, add 5 times the amount of 70% alcohol aqueous solution, heat and reflux to extract twice, each time for 2 hours, combine the extracts, filter, add 5 times the amount of filtrate (Volume ratio) 95% alcohol aqueous solution, let it stand overnight, centrifuge for 10 minutes (20000g), concentrate the supernatant under reduced pressure again, add 2% activated carbon and boil for 20 minutes, filter. The filtrate was adsorbed on the AmberliteXAD2 adsorption resin column, and then eluted with lower alcohols. Concentrate the eluate, perform ultrafiltration with a 0.4 μm filter membrane, pack in ampoules, 2ml each, freeze-dry and seal, check for air leakage, and then make lyophilized powder for injection.

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Abstract

The invention provides a compound injection of Radix Radix and Motherwort, which is prepared by the following method: Take Radix Radix Radix and Motherwort with a weight ratio of 1:2 to 1:10, heat and reflux extract with 20% to 80% ethanol, filter, Concentrate, centrifuge, and purify the supernatant through an adsorption resin column, sterile filter, and add sterile water for injection. The compound injection of isatidis root and motherwort of the present invention can treat cardiovascular and cerebrovascular diseases, especially coronary heart disease, angina pectoris or chest obstruction.

Description

technical field [0001] The invention relates to a traditional Chinese medicine compound injection, in particular to a compound injection of Radix Radix and Motherwort. Background technique [0002] "Chinese herbal medicine" magazine, No. 9, 2003, page 777, introduces the preparation method of Radix isatidis ethanol extract. 10 kg of Radix isatidis, cut into small pieces, extracted three times with 95% ethanol under continuous reflux, recovered ethanol, dispersed the extract with appropriate amount of water, and extracted with petroleum ether (60-90°C), chloroform, ethyl acetate and n-butanol in sequence. The ethyl acetate part was chromatographed on a silica gel column and eluted with a gradient of chloroform-methanol, with 500 ml as a fraction, and the 203-221 fractions were combined and then recrystallized by chloroform-methanol to obtain compound I. Fractions 80-94 were combined and prepared by high performance liquid phase, and eluted with 35% methanol to obtain compoun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/533A61K36/19A61K36/315A61K9/19A61K9/08A61P9/00A61P9/10A61P7/02
Inventor 于荣敏
Owner 于荣敏