Method for inducing female nucleus growth with heterologous frozen sperm

A technique for gynogenesis and spermatogenesis for use in fish sex control and production of all-female seed
CN100539832CInactive Publication Date: 2009-09-16YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Publication Date
2009-09-16
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

A method for inducing gynogenesis by heterologous frozen sperm of fish, including cryopreservation and genetic inactivation of heterologous fish sperm, and a method for inducing gynogenetic diploid fry. The present invention establishes for the first time a method for inducing gynogenetic development of various seawater fish eggs by adopting the frozen sperm of perch, and screens out the cold shock initiation time and cold shock time suitable for the eggs of spotted flounder, turbot and flounder, respectively. Temperature and duration of cold shock, induction of gynogenetic diploid fry established. The percentages of gynogenetic fry obtained by adopting the method of the invention are 7.8-40.7%, 26-34% and 10-25% respectively. The method established by the invention has the characteristics of being advanced, efficient, accurate and reliable, has important application value in fish sex control and all-female fry production, and has broad promotion and application prospects in fish breeding and breeding.
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Description

Technical field:

[0001] The invention belongs to fish genetic breeding technology, and is a method for cultivating fish gynogenetic diploids by using heterologous frozen sperm, which can be applied to fish sex control and production of all-female seedlings. Background technique:

[0002] Male and female differences in growth rate exist in many fish species. For example, tilapia males grow more than 50% faster than females, and many marine flounder females grow much faster than males, such as star flounder and Atlantic flounder. Individuals are 50-100% faster. Due to the slow growth of male individuals, the aquaculture yield and aquaculture benefits of these flounder fishes have been reduced, the aquaculture cost has been increased, and the promotion of these fish seedlings and the formation of aquaculture industry have been seriously affected. The research on nuclear development technology, the development of all-female populations, and the promotion and application of all...

Claims

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