Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for inducing female nucleus growth with heterologous frozen sperm

A technique for gynogenesis and spermatogenesis for use in fish sex control and production of all-female seed

Inactive Publication Date: 2009-09-16
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports on gynogenesis induced by heterologous frozen sperm in fish at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing female nucleus growth with heterologous frozen sperm
  • Method for inducing female nucleus growth with heterologous frozen sperm
  • Method for inducing female nucleus growth with heterologous frozen sperm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0042] Using genetically inactivated heterologous frozen sperm to stimulate eggs, and at the same time, cold-shocking gynogenetic eggs, inhibiting the second polar body from expelling and doubling chromosomes, can successfully induce spotted flounder, Atlantic flounder and turbot, etc. For the gynogenesis of flounder and flounder, the gynogenetic induction technology induced by heterologous frozen sperm that is commonly used in seawater fish is established. For the sex control of these flounder and the cultivation of all-female parthenogenetic seedlings, all-female It has important practical significance and great potential for popularization and application to raise the breeding yield and economic benefits of these fish species.

[0043] Take the spotted flounder and turbot as examples below to describe the technical content of the present invention in detail:

[0044] The technical content of the invention includes three aspects: 1. genetic inactivation of the frozen sperm o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
induction rateaaaaaaaaaa
induction rateaaaaaaaaaa
induction rateaaaaaaaaaa
Login to View More

Abstract

A method for inducing gynogenesis by heterologous frozen sperm of fish, including cryopreservation and genetic inactivation of heterologous fish sperm, and a method for inducing gynogenetic diploid fry. The present invention establishes for the first time a method for inducing gynogenetic development of various seawater fish eggs by adopting the frozen sperm of perch, and screens out the cold shock initiation time and cold shock time suitable for the eggs of spotted flounder, turbot and flounder, respectively. Temperature and duration of cold shock, induction of gynogenetic diploid fry established. The percentages of gynogenetic fry obtained by adopting the method of the invention are 7.8-40.7%, 26-34% and 10-25% respectively. The method established by the invention has the characteristics of being advanced, efficient, accurate and reliable, has important application value in fish sex control and all-female fry production, and has broad promotion and application prospects in fish breeding and breeding.

Description

Technical field: [0001] The invention belongs to fish genetic breeding technology, and is a method for cultivating fish gynogenetic diploids by using heterologous frozen sperm, which can be applied to fish sex control and production of all-female seedlings. Background technique: [0002] Male and female differences in growth rate exist in many fish species. For example, tilapia males grow more than 50% faster than females, and many marine flounder females grow much faster than males, such as star flounder and Atlantic flounder. Individuals are 50-100% faster. Due to the slow growth of male individuals, the aquaculture yield and aquaculture benefits of these flounder fishes have been reduced, the aquaculture cost has been increased, and the promotion of these fish seedlings and the formation of aquaculture industry have been seriously affected. The research on nuclear development technology, the development of all-female populations, and the promotion and application of all...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00C12N5/06C12N15/06C12Q1/68A61D19/02C12N5/075
CPCY02A40/81
Inventor 陈松林杨景峰苏鹏志翟介明田永胜廖小林邵长伟
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com