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Tissue culture quick breeding method of orange red lamp stage primula

A technology of tissue culture rapid propagation and lampstand, which is applied in the field of plant tissue culture, can solve the problems that there is no primula in orange red lampstand, and the method of division propagation cannot meet the production demand, etc., and achieve shortening the seedling cycle, strong consistency, The effect of easy seedling growth

Inactive Publication Date: 2009-11-18
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to seed propagation, the available methods for asexual propagation of Primula are: branch (leaf cluster) propagation, tissue culture propagation, and a small number of perennial species can be propagated by cuttings, but the method of branch propagation is far from meeting production needs.
Tissue culture is an important way of rapidly propagating good plant varieties, and many species of Primula have carried out relevant reports on tissue culture, but there is no relevant research and report on the tissue culture of Primula bullleyana (Primulabulleyana), so It is necessary to study the tissue culture of Primula bulleyana and form a special rapid propagation technology system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The leaves of Primula bulleyana (Primula bulleyana) were used as explants to explore the optimal medium for induction: the leaves of well-growing seedlings were used as explants to explore the optimal medium for induction. Wash the young leaves of Primula bulleyana with tap water for 2-3 hours, drop a drop of detergent in the water and shake for 15 minutes to remove surface impurities, and rinse with tap water repeatedly; surface disinfection: 70% alcohol disinfection for 10-15 seconds, without Rinse with sterile water for 4 to 5 times, disinfect with 0.1% mercuric chloride solution for 3 to 4 minutes, and then rinse with sterile water for 4 to 5 times. The sterilized leaves were then cut into 1 cm 2 Small pieces (with part of the veins) were inoculated on the induction medium of clustered buds, 5-6 pieces were inoculated in each bottle, and 10 bottles were connected to each medium, and cultured in the dark. Among them, the concentration gradient of 6-BA: 0.5mg / l, 1.0m...

Embodiment 2

[0026] The axillary buds of Primula bulleyana (Primula bulleyana) were used as explants for primary culture and subculture: select axillary buds from robust plants, rinse them with tap water for 2-3 hours, disinfect and sterilize them in the same way as leaves, and inoculate them on MS+ 6-BA 2.0mg / l+NAA 0.1mg / l cluster bud induction medium, inoculate 2-3 buds per bottle; the light conditions are natural scattered light 3000Lux plus artificial auxiliary light source 1800±200Lux, light time 14h.

[0027] When the axillary buds are inoculated on the differentiation medium, new leaves are continuously pulled out. From 10 days on, the petioles of the new leaves are thick and the base turns red, and light green callus is gradually formed. After 2 weeks, adventitious buds begin to differentiate; 40 days after inoculation , A large number of adventitious buds are differentiated on the newly drawn petioles and leaves of the axillary buds.

[0028] The statistical results show that afte...

Embodiment 3

[0030] Rooting culture of tissue-cultured seedlings of Primula bulleyana: when the adventitious buds grow 2 to 3 leaves on the differentiation medium, they are excised from the callus and transferred to the rooting medium, formula : In MS, MS+NAA (0.1mg / l, 0.2mg / l, 0.5mg / l), the pH is 5.8; the light conditions are natural scattered light 3000Lux plus artificial auxiliary light source 2500Lux, light time 14h.

[0031] From the perspective of the time required for rooting, the time required for rooting in medium MS and MS+0.2mg / l NAA is the shortest, 8-10 days, but in MS+0.2mg / l NAA, the rooting amount of tissue culture seedlings And growth is obviously better than MS basic medium, the average height of tissue culture seedlings can reach 4.8cm, and the rooting rate is over 95%.

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Abstract

The invention provides a method for tissue culture and rapid propagation of Primula citrus, which comprises explant selection, surface disinfection, induced culture, subculture, rooting culture and transplanting. According to the tissue culture rapid propagation method of Primula bulleyana of the present invention, one axillary bud can be induced to produce about 40 to 50 new buds, and the multiplication coefficient is 3 to 4 after about 30 to 40 days of subculture, and the rooting rate can reach It has reached more than 95%, and the survival rate of transplanting is almost 100%. It has realized the preservation of a single plant of the excellent Primula bulleyana through rapid propagation technology, and at the same time greatly improved the seedling production of Primula bulleyana ability, shorten the seedling time and reduce the cost, and provide technical support for the large-scale promotion and application of primrose.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a tissue culture rapid propagation method of Primula bulleyana. Background technique [0002] Primula bulleyana (Primula bulleyana) is a plant of the genus Primula in the Primulaceae family. It is a very beautiful garden ornamental flower. Primula bulleyana is a perennial herbaceous flower. The whole plant is in the shape of a rosette, with well-developed rhizomes and 5 or more leaf clusters growing upwards. It is mainly propagated by seeding. Generally, after sowing in spring, it grows vegetatively It begins to flower in May of the following year, grows slowly, and has a low reproduction coefficient. In addition to seed propagation, the available methods for asexual propagation of Primula are: branch (leaf cluster) propagation, tissue culture propagation, and a small number of perennial species can be propagated by cuttings, but the method of branch propagation is far from meeting produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G1/00A01G7/00
Inventor 张启翔李翠娟潘会堂梁树乐程堂仁孙明
Owner BEIJING FORESTRY UNIVERSITY
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