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Method for detecting content of paeoniflorin, psoralen and isopsoralen in pill for strengthening spleen and intestines

A technology of isopsoralen and psoralen, which is applied in the field of quality standard detection of Bupi Yichang Pills, can solve the problems of inability to fully reflect the quality of drugs and low technical content

Active Publication Date: 2009-12-23
GUANGZHOU BAIYUNSHAN CHENLIJI PHARMA FAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The original quality inspection methods only have microscopic identification and thin-layer chromatography identification, which cannot fully reflect the quality of the drug, and the technical content is low

Method used

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  • Method for detecting content of paeoniflorin, psoralen and isopsoralen in pill for strengthening spleen and intestines
  • Method for detecting content of paeoniflorin, psoralen and isopsoralen in pill for strengthening spleen and intestines
  • Method for detecting content of paeoniflorin, psoralen and isopsoralen in pill for strengthening spleen and intestines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Example 1 (Bupi Yichang Pills: provided by Chen Liji Pharmaceutical Factory, batch number: B06012)

[0094] [Content Determination] Psoraleae Radix Paeoniae Alba was determined according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2005 Edition).

[0095]Chromatographic conditions and system suitability test Octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, water is used as mobile phase B, and the wavelength is changed for detection, and the gradient elution detection is carried out in the following table. The number of theoretical plates should not be less than 2000 based on the peak of paeoniflorin.

[0096]

[0097] Preparation of Reference Substance Solution Accurately weigh the appropriate amount of paeoniflorin reference substance, psoralen reference substance, and isopsoralen reference substance, and add methanol to make each 1ml contains 30 μg of paeoniflorin, 4 μg of psoralen, and is...

Embodiment 2

[0101] Example 2 (Bupi Yichang Pills: provided by Chenliji Pharmaceutical Factory, batch number: B06012)

[0102] [Content Determination] Psoraleae Radix Paeoniae Alba was determined according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2005 Edition).

[0103] Chromatographic conditions and system suitability test Octadecylsilane-bonded silica gel is used as filler; acetonitrile is used as mobile phase A, water is used as mobile phase B, and wavelength-variable detection is performed by gradient elution detection as shown in the table below. The number of theoretical plates should not be less than 2000 based on the peak of paeoniflorin.

[0104]

[0105] Preparation of Reference Substance Solution Accurately weigh the appropriate amount of paeoniflorin reference substance, psoralen reference substance, and isopsoralen reference substance, and add methanol to make each 1ml contains 30 μg of paeoniflorin, 4 μg of psoralen, and isopsoralen...

Embodiment 3

[0109] Example 3 (Bupi Yichang Pills: provided by Chen Liji Pharmaceutical Factory, batch number: B06012)

[0110] [Content Determination] Radix Paeoniae Alba and Psoraleae were determined according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2005 Edition).

[0111] Chromatographic conditions and system suitability test Octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, water is used as mobile phase B, and the wavelength is changed for detection, and the gradient elution detection is carried out in the following table. The number of theoretical plates should not be less than 2000 based on the peak of paeoniflorin.

[0112]

[0113] Preparation of Reference Substance Solution Accurately weigh the appropriate amount of paeoniflorin reference substance, psoralen reference substance, and isopsoralen reference substance, and add methanol to make each 1ml contains 30 μg of paeoniflorin, 4 μg of psoralen, ...

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Abstract

The invention discloses a quality detection method of Bupi Yichang Pills, which uses high-performance liquid chromatography for gradient elution and simultaneously measures the contents of paeoniflorin, psoralen and isopsoralen. The purpose of the present invention is to provide a quality detection method of Bupi Yichang Pills. The effective components of paeoniflorin, psoralen and isopsoralen in the inner and outer layers of the drug are separated by high-performance liquid chromatography gradient elution, so that the active components can be obtained most accurately under the conditions of the best mobile phase and the maximum absorption wavelength. inspection.

Description

technical field [0001] The invention relates to a quality standard detection method of Bupi Yichang pills, in particular to a method for detecting the contents of paeoniflorin, psoralen and isopsoralen in Bupi Yichang pills. technical background [0002] Bupi Yichang double-layer pill is the first patented product (application (patent) number: 87106202) of our factory. It is prepared by the process of gastrointestinal separation and has remarkable curative effect. [0003] The original quality inspection methods only have microscopic identification and thin-layer chromatography identification, which cannot fully reflect the quality of the drug, and the technical content is low. Through the research on the inner and outer layers of the drug, we have invented a detection method with gradient elution and changing the detection wavelength by applying high performance liquid chromatography. The advantage of this method is that the content of active ingredients in the inner and ou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02A61K36/71A61K36/487A61P1/00
Inventor 王姗谢琳
Owner GUANGZHOU BAIYUNSHAN CHENLIJI PHARMA FAB CO LTD