Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Offspring-parent discrimination method for Chinese Hesitan milk cow and its special-use primer and kit

A paternity test, dairy cow technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problems of poor effectiveness and low accuracy of identification results, and achieve the effect of simple use, reliable test results and high accuracy

Inactive Publication Date: 2010-02-03
CHINA AGRI UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effectiveness of traditional serum protein markers and blood group factors for dairy cow parentage identification is poor, and the accuracy of identification results is low. It is urgent to use new genetic markers to effectively solve this problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Offspring-parent discrimination method for Chinese Hesitan milk cow and its special-use primer and kit
  • Offspring-parent discrimination method for Chinese Hesitan milk cow and its special-use primer and kit
  • Offspring-parent discrimination method for Chinese Hesitan milk cow and its special-use primer and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Screening of microsatellite primers for paternity testing on Chinese Holstein dairy cows

[0039] The selection of microsatellite markers

[0040] According to the existing literature and related research results of others, a total of 30 microsatellite markers recommended by FAO were selected, and their full nucleotide sequences, primer sequences, PCR reaction conditions, population polymorphism information and chromosomal genetic map positions can be used. Obtained from: http: / / www.ncbi.nlm.nih.gov / , http: / / www.marc.usda.gov, http: / / www.ri.bbsrc.ac.uk. The principle of marker selection is that the number of alleles is large, with a high degree of polymorphism, and there is no linkage relationship between them, and they are distributed in multiple different chromosomes. The specific information of the selected 30 microsatellite markers is shown in Table 1:

[0041] Table 1 Microsatellite marker information

[0042]

[0043]

[0044]

[0045]

...

Embodiment 2

[0109] Example 2. Verification of Microsatellite Detection System

[0110] Theoretically, as long as there is a genotype of a genetic marker that does not conform to Mendel's laws of inheritance, it can be concluded that patriarchal relationship is excluded. However, by detecting DNA polymorphisms to identify paternity, there is a possibility of false exclusion. In order to prevent false conclusions, the present invention follows the following principles in paternity exclusion: there are 3 or more microsatellite markers that do not conform to Mendelian The laws of heredity rule out parent-child relationship.

Embodiment 3

[0135] Example 3. Preparation of Chinese Holstein cow paternity test kit

[0136] The kit of the present invention for performing paternity testing on Chinese Holstein cows includes the following reagents:

[0137] (1) 2.5U / ul Taq DNA polymerase;

[0138] (2) 20× PCR amplification buffer;

[0139] (3) 25mmol / L 10×dNTPs;

[0140] (4) 25mmol / L MgCl 2 ;

[0141] (5) 12 pairs of special primers for the paternity test of Chinese Holstein dairy cows screened in Example 1, 25umol / L;

[0142] (6)ddH 2 O.

[0143] The specification of the kit is 25 times / box, and the amount of each component in each box is: 2.5U / ul Taq DNA polymerase 1 bottle (500ul / bottle), 20× PCR amplification buffer 1 bottle (500ul / bottle) , 25mmol / L 10×dNTPs 1 bottle (200ul / bottle), 25mmol / L MgCl 2 1 vial (500ul / vial), 1 vial (50ul / vial) of upstream and downstream primers for each pair of primers and ddH 2 O 1 bottle (1000ul / bottle). The components in the kit are packaged according to the above doses, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Chinese Holstein cow paternity testing method and the special primer and reagent box thereof. And the primer comprises the following primer pairs: 1) primer pair composed ofnucleotide sequences 1 and 2 in sequence list; 2) primer pair composed of nucleotide sequences 3 and 4; 3) primer pair composed of nucleotide sequences 5 and 6; 4) primer pair composed of nucleotide sequences 7 and 8; 5) primer pair composed of nucleotide sequences 9 and 10; 6) primer pair composed of nucleotide sequences 11 and 12; 7) primer pair composed of nucleotide sequences 13 and 14; 8) primer pair composed of nucleotide sequences 15 and 16; and 9) primer pair composed of nucleotide sequences 17 and 18.

Description

technical field [0001] The invention relates to a method for parent-child identification of Chinese Holstein dairy cows by using microsatellite marker technology, and special primers and kits thereof. Background technique [0002] Artificial insemination technology and genetic evaluation of breeding cattle are the most critical content in dairy cattle breeding, and have a huge impact on the genetic progress of dairy cattle. Among them, the genetic evaluation of bulls is the key and core content of dairy cattle breeding. Accurate genetic evaluation requires individual bulls. various relatives information. If the wrong pedigree is used, it will be difficult to accurately use the animal model BLUP for genetic evaluation of dairy cattle, which will cause serious losses in the selection of breeding bulls, the genetic progress of the population, and the overall production level. Amplify its influence in a population, thereby improving genetic progress. The use of artificial inse...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 张沅孙东晓田菲
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com