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Sample diluent in use for ELISA test and method for ELISA test

A diluent and basic technology, applied in the field of specimen diluent, can solve the problems of serum or plasma specimen diluent missing or wrongly added, and achieve the effects of long-term preservation, obvious color change, and obvious color reaction

Active Publication Date: 2010-02-10
CUSABIO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the shortcomings of missing or wrongly added serum or plasma sample diluents in the prior art, and provide a sample diluent that can be added accurately for ELISA tests

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The present invention provides a kind of diluent used in ELISA test, described diluent comprises calf serum as 2% by volume percentage, 0.7% antiseptic Proclin300, 0.0016% indicator bromine pink, and the remainder saline.

[0015] The method for using the diluent in the ELISA test includes diluting the test sample, incubation, adding enzyme-labeled antibody, adding substrate solution for color development and terminating the reaction steps, and the described step of diluting the test sample is calculated by volume percentage as 2% calf serum, 0.7% preservative Proclin300, and 0.0016% indicator bromine pink were added to the basic diluent physiological saline to prepare a sample dilution. The dilution was yellow, and then the dilution was added to the sample to be tested. Diluted in the specimen, it is pink in color. According to this method, adding errors or missing additions can be avoided.

[0016] Method described in the present invention can be applied in followin...

Embodiment 2

[0025] The present embodiment only illustrates the parts different from the embodiment, and the diluent is calculated as 1.5% calf serum, 0.5% preservative Proclin300, 0.0014% indicator bromine pink by volume percentage, and the remaining amount saline composition.

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PUM

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Abstract

A sample diluting solution used in ELISA test is prepared from 0.0014-0.0017 volume% of indicator and allowance of basic diluting solution. It is featured as adding calf serum and indicator into basicdiluting solution then diluting serum sample.

Description

technical field [0001] The invention relates to a sample diluent for ELISA test and a method for ELISA test. Background technique [0002] ELISA is the abbreviation of Enzyme-Linked Immunosorbnent Assay (Enzyme-Linked Immunosorbnent Assay), which is an enzyme immunoassay developed after immunofluorescence and radioimmunoassay. Due to the characteristics of sensitivity, specificity, economy, simplicity, and safety, ELISA test method is currently the most commonly used detection method in immunology laboratories. In ELISA tests, serum or plasma is the most commonly used specimen for various types of ELISA tests and other tests to detect various antigens or antibodies in blood, because ELISA tests often detect multiple specimens at the same time, and because the method is sensitive , for accurate determination, it is often necessary to dilute the samples to be tested in different proportions. In order to simplify the operation steps and save reagents, the samples can be direct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/38G01N33/53G01N21/78
Inventor 谷学英侯林浦牛向兰何欢曾序春
Owner CUSABIO TECH LLC