Polynucleotides encoding mature AHASL proteins for creating imidazolinone-tolerant plants

A polynucleotide and protein technology, applied in the field of plant molecular biology, can solve problems such as nucleotide and amino acid sequences that have not been reported
CN101006178AInactive Publication Date: 2007-07-25BASF PLANT SCI GMBH

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BASF PLANT SCI GMBH
Publication Date
2007-07-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

Isolated polynucleotide molecules that encode mature, wild-type and imidazolinone-tolerant acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences encoding these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotide molecules of the invention, as well as plants and host cells transformed with the polynucleotide molecules, expression cassettes, and transformation vectors, are described. Methods of using the polynucleotide molecules to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of such plants are also described.
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Description

Technical field

[0001] The present invention relates to the field of plant molecular biology, and more specifically to a new nucleotide sequence that encodes a wheat acetohydroxy acid synthase large subunit enzyme (hereinafter referred to as AHASL) and can be used to establish herbicide tolerance in plants. Background technique

[0002] Acetohydroxy acid synthase (AHAS; EC 4.1.3.18, also called acetolactate synthase or ALS), is the first enzyme that catalyzes the biochemical synthesis of branched-chain amino acids valine, leucine and isoleucine ( Singh (1999) "Biosynthesis of valine, leucine and isoleucine," in Plant Amino Acid, Singh, BK, ed., Marcel Dekker Inc. New York, New York, pp. 227-247). AHAS is the action site of 4 different herbicide families in structure, including sulfonylurea (LaRossa and Falco (1984) Trends Biotechnol. 2:158-161), imidazolinone (Shaner et al. (1984) Plant Physiol) .76:545-546), triazolopyrimidines (Subramanian and Gerwick (1989) "Inhibition of acet...

Claims

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