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Method for extracting effective sites group of smilax China root

A technology of effective parts and smilax, which is applied in medical preparations containing active ingredients, pharmaceutical formulas, extracellular fluid diseases, etc., can solve the waste of medicinal resources, the low extraction efficiency of saponins and flavonoids, and the impurities in extracts. high content problem

Inactive Publication Date: 2007-08-08
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the smilax preparations (syrup, capsule, etc.) prepared from smilax as raw materials all use water extraction and alcohol precipitation process, the extraction efficiency of saponins and flavonoids is low, and the content of impurities in the extract is high, resulting in waste of medicinal material resources and large dosage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Weigh 1000g of sarsaparilla coarse powder, use 6 times the amount of 60% ethanol to reflux extract twice, each time for 3 hours, filter, combine the filtrate, recover the ethanol to a certain volume, adjust the pH value to 2 with hydrochloric acid solution as the column liquid.

[0014] Pass the upper column liquid through the adsorption column filled with HPD100 type macroporous adsorption resin at a flow rate of 0.5 times the volume of the adsorption material column per hour, rinse with water until it is colorless, then elute with 3 times the amount of 10% ethanol, discard the washed Deliquification, then eluting with 8 times the amount of 40% ethanol, collecting the eluate, reclaiming the ethanol under reduced pressure, concentrating to dryness, obtaining a brown extract, weighing 61 grams, and the yield is 6.1%. Its total saponins and total The sum of flavonoid content is 52.3%.

Embodiment 2

[0016] Weigh 1000g of sarsaparilla coarse powder, use 8 times the amount of 80% ethanol to reflux extract twice, each time for 1 hour, filter, combine the filtrate, recover the ethanol to a certain volume, adjust the pH value to 3 with hydrochloric acid solution as the column liquid.

[0017] The upper column liquid is passed through the adsorption column filled with XDA-6 macroporous adsorption resin at a flow rate of 2 times the volume of the adsorption material column per hour, washed with water until it is colorless, then eluted with 5 times the amount of 20% ethanol, and discarded. Remove the eluate, then elute with 15 times the amount of 50% ethanol, collect the eluate, concentrate to dryness, and obtain a brown extract, weighing 58 grams, and the yield is 5.8%. The content of its total saponins and total flavonoids The sum is 57.1%.

Embodiment 3

[0019] Weigh 1000g of sarsaparilla coarse powder, use 10 times the amount of 50% ethanol to reflux extract three times, each time for 2 hours, filter, combine the filtrate, recover the ethanol to a certain volume, adjust the pH value to 4 with hydrochloric acid solution as the upper column liquid .

[0020] Pass the upper column liquid through the adsorption column filled with D101 type macroporous adsorption resin at a flow rate of 1.5 times the volume of the adsorption material column per hour, rinse with water until it is colorless, then elute with 3 times the amount of 20% ethanol, discard the washed Deliquification, then elute with 10 times of 70% ethanol, collect the eluent, concentrate to dryness, and obtain a brown extract, weighing 63 grams, with a yield of 6.3%, the sum of its total saponins and total flavonoids was 60.7%.

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Abstract

The invention discloses a process for preparing significant portion groups of smilax China root which comprises the following steps: (1) disintegrating smilax China root rhizome, charging smilax China root coarse powder into 30-80% ethanol solution for backflow extraction, merging the filter liquors, reclaiming ethanol till no alcohol smell is present, adjusting pH of the extract to 2-6 with hydrochloric acid, passing the extract through nonpolar or polar macroscopic adsorption resin column bed, purifying the resin bed to achromatic color with water, washing resin columns with three times of 10-39% ethanol solution, discarding the eluent, eluting with 8-15 times of 40-90% ethanol solution, collecting the eluent, finally carrying out compensation. The obtained significant portion groups can be used for preparing compositions for the treatment of gynaecologic inflammation, tumor, cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and relates to a method for preparing effective fractions of smilax. The method uses a macroporous adsorption resin to prepare total saponins and total flavonoids effective fractions from smilax. Background technique [0002] Smilax is a commonly used traditional Chinese medicine. It was first recorded in "Famous Doctors", and now it is recorded in the Chinese Pharmacopoeia 2005 edition. Its medicinal part is the dried rhizome of Smilax China L., a plant of Liliaceae. Smilax is sweet, slightly bitter, flat in nature, and belongs to the liver and kidney channels. It has the effects of dispelling rheumatism, diuresis, detoxification and dispelling blood stasis. It is used for muscle and bone pain, profuse urination, profuse vaginal discharge, furuncle carbuncle, scrofula, etc. , its total extract preparations have been widely used in gynecological chronic inflammation, adnexit...

Claims

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Application Information

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IPC IPC(8): A61K36/90A61P29/00A61P7/10A61P15/00A61P17/02A61K125/00
Inventor 叶晓川李文霞颜彦杨祥良
Owner HUAZHONG UNIV OF SCI & TECH
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