Immunostimulator, method and apparatus for producing the same
An immune enhancer, immune function technology, used in chemical instruments and methods, pharmaceutical formulations, inorganic active ingredients, etc.
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no. 1 Embodiment
[0056] In this embodiment, the electrolytic alkaline water generated in the generating device of the immune enhancer is used to electrolyze the silver components of different concentrations to generate alkaline water (test drug), in order to confirm its inhibitory effect on tumor cell proliferation , and the antitumor effect on transplanted tumors, the following two in vivo tests (in vitro) were carried out.
[0057] (1) Experiment 1: Effects on various tumor cells
[0058] In this experiment, various mouse and human cancer cells cultured and passaged in the laboratory of Kitasato Research Institute were used as test materials. In addition, as test drugs, four types of electrolytically produced alkaline water (test drugs 1 to 4) produced at a time in the immunopotentiator producing device were used. Test drug 1 is electrolyzed alkaline water with pH 9.3 and silver concentration 50 ppb, test drug 2 is electrolytic alkaline water with pH 9.3 and silver concentration 25 ppb, tes...
no. 2 Embodiment
[0096] In the present embodiment, the electrolytic generation of alkaline water generated in the generating device of the immune enhancer is used, and the electrolysis of silver components of different concentrations is used to generate alkaline water (test drug). 180. The antitumor effect of Meth-A) was carried out in the following two experiments (in vitro). The tumors used in this example were S-180 tumors and Meth-A tumors cultured and preserved in the laboratory of Kitasato Research Institute. In addition, as the test drug, the electrolysis-generated alkaline water (test drug 1-4) with different silver component concentrations generated by the immunopotentiator generating device was used. Distilled water was used as a negative control, and versicolor polysaccharide pSK (positive control: manufactured by Sankyo Co., Ltd.) was used as a positive control. The test drug 1 is electrolyzed alkaline water (called Ag0 reagent) with a pH of 9.5 and a silver ion concentration of 0...
no. 3 Embodiment
[0120] In this example, the effects of electrolytically generated alkaline water, distilled water (control) and PSK (positive control) with different silver component concentrations generated in the immunopotentiator generating device on T lymphocytes were confirmed through experiments.
[0121] In this experiment, the spleen removed from the mouse (day 24) administered with each reagent was flattened on a microscope slide to prepare a cell suspension, which was superimposed on (Lympholite-M) at 20°C at a rate of 2000rpm Perform centrifugation for 20 minutes to obtain T cells. The obtained T cells were suspended in MEM medium and adjusted to 1×10 6 Cells / ML / well, as antigen, add 50μL of (S-180) membrane differentiation, in 5% CO 2 The culture was carried out at 37° C. for 24 hours in the presence of the cells. After incubation, react with FITC-labeled CD3e antibody and PE-labeled CD233 antibody. After washing, the activation of lymphocytes was measured by FACS (flow cytomet...
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