Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for inducing liver cell from human embryonic stem cells

A technology of human embryonic stem cells and liver cells, which is applied in the field of directed differentiation of human embryonic stem cells in vitro, can solve the problems that mouse-derived liver-like cells cannot be applied, and achieve the effects of flexible timing, easy identification, high reliability and accuracy

Inactive Publication Date: 2007-09-19
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE +1
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to ethical and transplant rejection considerations, the induced mouse liver-like cells cannot be used in humans at all.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing liver cell from human embryonic stem cells
  • Method for inducing liver cell from human embryonic stem cells
  • Method for inducing liver cell from human embryonic stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] During the culture process of human embryonic stem cells, when the cells grow in suspension, they will aggregate into spherical cell clusters, which are called embryoid bodies (embryoid bodies, EBs). It has been found that the development and differentiation process of EB cells is surprisingly similar to that of normal embryos (Keller et al., CurrOpin Cell Biol. 1995, 7: 862-869; Loebel et al., Dev. Biol. 2003, 264: 1-14). Therefore, this is regarded as a model for simulating embryonic development in vitro, on the basis of which, the possibility of embryonic stem cells to develop and differentiate into various types of cells and its process can be deeply studied. The study on the differentiation of embryonic stem cells into hepatocytes in the present invention is just based on this basis.

[0037] Dimethyl sulfoxide (DMSO) is a 2 h 6 Compounds of OS. In the cell differentiation system in vitro, it is reported that DMSO can promote the differentiation to mesoderm (Mum...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method of inducing human embryon stem cells as liver cells out of body via embryoid bodies using DMSO as dissmilation starter solution and allying nourish factors of liver cell containing niacinamide. The liver cells obtained by the method is also provided, which originating from human embryon stem cells with conformation as normal liver cell and subcellular structure as bile capillary, gene products for expressing differentia of the liver cell such as G6PC, LST, CPS1, CYP7A1, FIX etc., and can perform function of mature liver cells. The invention also provides such liver cells having founctions as liver cells or liver transpalnt, liver sick treating, artifical liver forming, medicine identifying and selecting, toxicity testing and toxicity research.

Description

technical field [0001] The invention relates to the fields of developmental biology, cell biology and tissue engineering, and more specifically relates to the in vitro directed differentiation of human embryonic stem cells. The invention provides a new method for inducing embryonic stem cells into hepatocytes in vitro, the hepatocytes obtained by the method, and the use of the hepatocytes in liver and hepatocyte transplantation, in vitro artificial liver and biological liver construction, drug identification and screening , drug toxicity determination and toxicology research, and the use in the prevention, diagnosis and treatment of liver diseases. Background technique [0002] The liver is a very important organ in the human body. It is not only the largest digestive gland, but also an important organ that metabolizes substances and produces a variety of biologically active substances. The incidence of liver disease in my country is very high, especially the incidence of l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/08C12N5/071C12N5/0735
Inventor 王茜李瑞珍盛慧珍
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com