Iso chicoric acid and extraction separation method thereof and usage in medicament

A water extraction, Compositae plant technology, applied in the field of extraction and separation of effective parts of traditional Chinese medicine and medical applications

Inactive Publication Date: 2007-10-31
高其品 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The isocichoric acid (scientific name: two-3,5-dihydroxycinnamoyl tartaric acid) involved in the present invention is a new compound extracted and separated from the Compositae plant Sophora pulmonica. After CA retrieval, isocichoric acid is a kind of New compound, so far, no report on extraction, separation and medical use

Method used

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  • Iso chicoric acid and extraction separation method thereof and usage in medicament
  • Iso chicoric acid and extraction separation method thereof and usage in medicament
  • Iso chicoric acid and extraction separation method thereof and usage in medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Preparation of Di-3,5 Dihydroxycinnamoyl Tartaric Acid

[0069] Take 100kg of the medicinal material Amulinia acuminata, add 15 times the amount of water, decoct twice for 2 hours each time, combine the filtrate, concentrate to contain 10 grams of medicinal material per ml, add ethanol to the alcohol concentration of 60%, centrifuge, and supernatant 10% calcium oxide milk was added to the solution to adjust the pH to 8-9, and 10 kg of precipitate was obtained. The precipitate was suspended in 90% ethanol, adjusted to pH = 3 with sulfuric acid, and centrifuged. The alcohol solution was adjusted to pH = 5 with 40% sodium hydroxide solution. -6. Centrifuge, recover ethanol, add water to dilute to a concentration of 10g of crude drug per ml, pass through the treated polyamide column, elute with distilled water and 60% ethanol successively, collect each part of the eluate, and concentrate the water eluate appropriately , extracted with ethyl acetate, discarded the ethyl acet...

Embodiment 2

[0071] Preparation of Di-3,5 Dihydroxycinnamoyl Tartaric Acid

[0072] Take 100kg of the medicinal material Amulinia acuminata, add 10 times the amount of water, decoct twice for 1.5 hours each time, combine the filtrates, concentrate to contain 20 grams of medicinal materials per ml, add ethanol until the alcohol concentration is 700%, centrifuge, and supernatant Add 10% calcium oxide milk to the solution to adjust the pH to 9-10, obtain 12 kg of precipitate, centrifuge, adjust the pH of the alcoholic solution to 6-7 with 40% sodium hydroxide solution, centrifuge, recover ethanol, add water to dilute to contain 10 g of crude drug per ml Through the treated macroporous resin column, elute with distilled water and 60% ethanol successively, collect the water eluate, concentrate it properly, precipitate the concentrated solution with acetone, dissolve the precipitate with water, add methanol for precipitation, and dissolve the precipitate with water Recrystallized with methanol t...

Embodiment 3

[0074] Preparation of Di-3,5 Dihydroxycinnamoyl Tartaric Acid

[0075] Take 100kg of the medicinal material Amulinia acuminosa, add 20 times the amount of water, decoct 3 times, 2 hours each time, combine the filtrate, concentrate to contain 10 grams of medicinal material per ml, add ethanol to the alcohol concentration of 80%, centrifuge, and supernatant Add 10% calcium oxide milk to the liquid to adjust to PH=10-12, obtain 8kg of precipitate, centrifuge, adjust pH=6-7 with 40% sodium hydroxide solution, centrifuge, reclaim ethanol, add water to dilute to contain 15g crude drug per ml concentration, mixed into silica gel, packed into a column, developing agent: n-butanol: acetic acid: water = 70:35:10, developed, cut and collected according to the color band, dissolved in methanol, and recrystallized repeatedly with water and methanol to obtain More than 80% di-3,5 dihydroxycinnamoyl tartaric acid compound 360mg.

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Abstract

The invention discloses a new compound -iso chicoric acid, which comprises the following steps: water-extracting alcohol deposition for embrace stem gutweed vegetable; centrifuging; adding burnt lime into supernatant fluid; depositing with pH value at 9-12; centrifuging; depositing; adding into alcohol to float; adding into sulfuric acid to adjust pH value at 1-4; centrifuging; reclaiming alcohol; proceeding polyamide or macroreticular resinous column chromatography; washing; eluting with diverse density alcohol; collecting eluent of each section; condensing; disposing with acetone, acetic ester and methyl alcohol; recrystallizing repeatedly; getting the product. This invention also discloses usages of iso chicoric acid.

Description

Technical field: [0001] The invention provides isochoric acid, a new compound isolated from A. brusselscens, and also discloses its extraction method and medical use, and belongs to the technical field of extraction and separation methods for effective parts of traditional Chinese medicines and medical use. technical background [0002] Astragalus is a perennial herb with a height of 30-50 cm. The whole plant is glabrous, the root is long conical, the surface is brown to tan, with longitudinal wrinkles and fibrous roots; the whole plant is used as medicine to treat headache, toothache, hematemesis, Epistaxis, dysentery, diarrhea, intestinal carbuncle, chest and abdominal pain, carbuncle sore swelling, trauma swelling and pain. The isocichoric acid (scientific name: two-3,5-dihydroxycinnamoyl tartaric acid) involved in the present invention is a new compound extracted and separated from the Compositae plant Sophora pulmonica. After CA retrieval, isocichoric acid is a kind of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732A61K31/216A61K36/28A61P35/00A61P37/02
Inventor 陈英红罗浩铭高阳姜瑞芝高其品
Owner 高其品
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