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Method for measuring human body red blood cell acidum folicum content

A determination method and technology for red blood cells, which are used in biological testing, material testing products, and analysis by chemical reaction of materials, etc., can solve the problems of long reaction time, large environmental pollution, poor stability of reagents, etc., and achieve stable test results. The effect of high sensitivity and simple operation

Active Publication Date: 2007-12-05
山东优生医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has cumbersome determination steps, long reaction time, and poor stability of reagents. More importantly, because the markers are radioactive isotopes, they pollute the environment and pose a threat to human health. world can only do it as a last resort

Method used

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Embodiment Construction

[0010] The human erythrocyte folic acid content determination method of the present specific embodiment comprises the following steps:

[0011] (1) Collect whole blood specimens; 1.1 Patients must be fasting; 1.2 Specimen tubes should use polyethylene plastic tubes. Blood samples must be free of lipemia and hemolysis; 1.3 Anticoagulant blood samples must be anticoagulated with heparin (15IU / mol); blood samples without anticoagulants must be completely coagulated naturally; 1.4 Whole blood samples must be tested within 8 hours , or stored frozen (-20°C) after aliquoting, but must be used within 7 weeks (thaw once at room temperature).

[0012] (2) Whole blood sample pretreatment: mix the whole blood sample with 1-1.5% ascorbic acid solution 20 times its volume, and leave it at room temperature for 1 hour for later use; completely destroy the red blood cells and release the folic acid component of the red blood cells completely; Get hemolyzed.

[0013] (3) Determination of fol...

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Abstract

The invention discloses a testing method of folic acid in the human erythron, which comprises the following steps: (1) gathering the full-blood specimen; (2) predisposing the full-blood specimen; breaking the erythron completely; releasing the folic acid in the erythron thoroughly; (3) using chemical luminous method to test the folic acid content of full blood; (4) conversing the folic acid content of erythron through the folic acid content of full blood and the known erythron pressure. The invention can calculate the folic acid content in the erythron through full blood folic acid content, which can replace traditional radiation immune testing method completely to complement the blank of erythron folic acid content.

Description

(1) Technical field [0001] The invention relates to a method for measuring folic acid content in human red blood cells, which belongs to the category of marker immunology. (2) Background technology [0002] For a long time, in clinical testing, the determination of folic acid content in human serum is generally used to determine whether the human body is deficient in folic acid. In recent years, studies have shown that the content of folic acid in red blood cells is 10 to 20 times that of folic acid in serum, and the content of folic acid in serum fluctuates greatly due to the influence of external factors such as diet and disease. The content of folic acid in red blood cells is rarely disturbed by external factors, and it can more truly reflect the concentration of folic acid in tissues. Merely measuring the serum folic acid content cannot meet the requirements of clinical medicine. In particular, it is found that the level of folic acid in red blood cells is of great valu...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/50
Inventor 文正伟宋筱亮
Owner 山东优生医疗科技有限公司