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Method for preparing soybean isoflavone aglycon

A technology for isoflavone aglycone and soybean saponin, which is applied in the field of preparation of soybean isoflavone aglycone, can solve the problems of less soybean isoflavone aglycone, few times of reproducible and repeated use, not paying attention to by-product soybean saponin, etc. cost saving effect

Inactive Publication Date: 2010-05-19
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The content of soybean isoflavone aglycone in natural products is very small, and the existing preparation methods of soybean isoflavone aglycone do not use appropriate methods to convert glycosides into aglycones, and do not pay attention to the by-product soybean saponin during the preparation process. The filler is more expensive and less renewable and reused

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 100 grams of soybean meal, extract it with 60% ethanol aqueous solution, and the volume ratio of liquid to material is 5:1. After soaking for 30 minutes, extract twice at 50°C under reflux in a water bath, each time for 90 minutes; the above extract is filtered, concentrated, added Dilute with distilled water, apply weakly polar macroporous resin, rinse with distilled water first, and then rinse with 50% ethanol aqueous solution; the eluted part of 50% ethanol aqueous solution is a mixture of soybean saponins and soybean isoflavones. After drying, mix the sample and put it on a polyamide column, first elute it with distilled water, this part contains a lot of saponin; then wash it with 60% ethanol-water solution, this part contains a lot of soybean isoflavones. Dry and reflux in ethanol-hydrochloric acid aqueous solution for 60 minutes (the weight ratio of ethanol and hydrochloric acid is 1:5); the hydrolyzate is concentrated to no alcohol, after degreasing, extract...

Embodiment 2

[0022] Take 100 grams of soybean meal, extract it with a 70% ethanol aqueous solution, the volume ratio of liquid to material is 8:1, soak for 30 minutes, and extract twice at 50°C under reflux in a water bath, each time for 90 minutes; the above extract is filtered, concentrated, added Dilute with distilled water, apply weakly polar macroporous resin, first elute with distilled water, and then elute with 50% ethanol-water solution; the eluted part of 50% ethanol water solution is a mixture of soybean saponins and soybean isoflavones. After drying, mix the sample and put it on a polyamide column, elute with distilled water, this part contains a lot of saponin; then wash it with 70% ethanol-water solution, this part contains a lot of soybean isoflavones. Dry, reflux in ethanol-hydrochloric acid aqueous solution for 60min (the weight ratio of ethanol and hydrochloric acid is 1:6); 6:1) elution, that is, the content is 70.98%.

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PUM

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Abstract

The invention discloses a method for preparing soybean isoflavone aglycone. It comprises following steps: water bath refluxing and extracting, decompressing and condensing, washing in weak polar largeporous resin column, water and water- ethanol gradient, washing in polyamide column, water and water- ethanol gradient, refluxing and hydrolysis, defatting, extracting with hydrochloric acid- ethanol, purifying in silica gel column and getting soybean isoflavone aglycone. The invention is characterized by high- purity product, no pretreatment for raw material, reusable disslovant and column filling, saved cost and soybean saponin- containing side product.

Description

technical field [0001] The invention relates to soybean isoflavone aglycone, in particular to a preparation method of soybean isoflavone aglycone. Background technique [0002] Isoflavones (isoflavones) are non-steroidal compounds containing aromatic rings and are a type of phytoestrogens. Scientists have found in research that soybean isoflavones extracted from soybeans have the effects of anti-oxidation, prevention of osteoporosis, Alzheimer's syndrome, arteriosclerosis, breast cancer, prostate cancer and other diseases; the by-products Soybean saponins have the functions of lowering blood fat, anti-oxidation, and immune regulation. In addition to the above health functions, they also have emulsifying activity, foaming property, and surface activity. They are widely used in food, medicine, and cosmetics. [0003] The content of soybean isoflavone aglycone in natural products is very small, and the existing preparation methods of soybean isoflavone aglycone do not use appr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/36
Inventor 杨红陈耕夫罗少洪张磊习志刚
Owner GUANGDONG PHARMA UNIV
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