Cactus polyoses and the distilling purification method and the application thereof

A technology of cactus polysaccharide and purification method, which is applied in the field of cactus polysaccharide and its extraction and purification, to achieve the effects of fast ultrafiltration, mild conditions, and energy saving

A technology of cactus polysaccharide and purification method, which is applied in the field of cactus polysaccharide and its extraction and purification, to achieve the effects of fast ultrafiltration, mild conditions, and energy saving

CN101095480AInactive Publication Date: 2008-01-02SOUTH CHINA UNIV OF TECH
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  • Cactus polyoses and the distilling purification method and the application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Use 500 grams of fresh cactus stems as raw materials, remove impurities, crush them, extract polysaccharides with aqueous solution, the ratio of water to material is 6:1, the extraction temperature is 70°C, and the extraction time is 2.2h; extraction once, 4000 rpm Under the conditions, centrifuge for 20min and separate to obtain 3050mL of supernatant.

[0029] (2) Filtrate with 400-mesh nylon filter cloth as pretreatment, and then filter with an ultrafiltration membrane with a molecular weight cut-off of 10000ku, the ultrafiltration pressure is 0.20MPa, the ultrafiltration temperature is 25°C, and the volume flow rate of the feed liquid is controlled at 0.05L / min, control the concentration 5 times, and get 600mL of concentrated extract.

[0030] (3) The above-mentioned concentrated extract solution was settled at 4° C. for 8 hours at an ethanol volume concentration of 55%, centrifuged and dried to obtain crude polysaccharide OP1, 2.42 grams, and the supernatant wa...

Embodiment 2

[0033] (1) Use 500 grams of fresh cactus stems as raw material, remove impurities, crush, extract polysaccharides with aqueous solution, water-material ratio is 6:1, extraction temperature is 75°C, extraction time is 2.5h; extraction is 1 time, same as in Example 1 Centrifuge to obtain 3050 mL of supernatant.

[0034] (2) Filtrate with 400-mesh nylon filter cloth as pretreatment, and then filter with an ultrafiltration membrane with a molecular weight cut-off of 10000ku, the ultrafiltration pressure is 0.20MPa, the ultrafiltration temperature is 25°C, and the volume flow rate of the feed liquid is controlled at 0.04L / min, control concentration 6 times, get concentrated extract 500mL.

[0035] (3) The above-mentioned concentrated extract solution was settled at 4° C. for 8 hours at an ethanol concentration of 60% by volume, centrifuged and dried to obtain crude polysaccharide OP1, 2.48 grams, and the supernatant was concentrated by ultrafiltration to a crude polysaccharide con...

Embodiment 3

[0038] (1) Take 500 grams of fresh cactus stems as raw material, remove impurities, crush them, extract polysaccharides with aqueous solution, water-material ratio is 6:1, extraction temperature is 80°C, extraction time is 3 hours; extraction is 1 time, centrifuged to obtain supernatant Liquid 3050mL.

[0039] (2) Filtrate with 400-mesh nylon filter cloth as pretreatment, and then filter with an ultrafiltration membrane with a molecular weight cut-off of 10000ku, the ultrafiltration pressure is 0.20MPa, the ultrafiltration temperature is 25°C, and the volume flow rate of the feed liquid is controlled at 0.045L / min, control the concentration 7 times, and get 450mL of concentrated extract.

[0040] (3) The above-mentioned concentrated extract was settled at 4° C. for 8 hours at an ethanol concentration of 65% by volume, centrifuged and dried to obtain crude polysaccharide OP1, 2.69 grams, and the supernatant was concentrated by ultrafiltration to a crude polysaccharide concentr...

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Abstract

The invention discloses a method for extracting cactus polysaccharide, which comprises following steps: extracting with water to remove foreign matter, disintegrating cactus stalk, centrifuging and getting supernatant, filtering, purifying and condensing the supernatant with super filtering membrane, depositing condensed solution to get polysaccharide at ethanol solution with different concentrations, removing protein through Sevag method, and de-coloring with active carbon. The invention is characterized by temperate reaction condition, little polysaccharide loss, fast super-filtering speed, short process, and saved energy consumption. The product is oxidation-inhibitive for diabetes complication, and it can be used to prepare diabetes anti-oxidation medicine. The invention provides a mew way for wild cactus utilization.

Description

technical field [0001] The invention relates to a chemical method, in particular to cactus polysaccharide and its extraction and purification method and application. Background technique [0002] Diabetes mellitus (DM) is a chronic, multiple common disease, often accompanied by complications. It is also regarded as the world's three major diseases along with tumors and cardiovascular diseases. With the improvement of people's living standards in our country, the number of diabetic patients is increasing year by year. According to WHO statistics, there are currently more than 150 million diabetics in the world, and it is expected to exceed 300 million by 2025, and it is predicted that diabetes will be prevalent in developing countries in the 21st century. [0003] If blood sugar control is not satisfactory in diabetic patients, acute and chronic complications may occur over time. Common chronic complications include diabetes combined with hypertension, diabetic heart disea...

Claims

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Application Information

Patent Timeline
02 Jan 2008
Publication
CN101095480A
IPC
A23L1/09; A23L1/28; A23L1/29; A61K36/33; A61P3/10; A61K135/00; A23L33/105; A23L33/125
Inventors
杨宁; 赵谋明