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Cucumber female deseription related molecule label and uses thereof

A molecular marker, cucumber technology, applied in the application field of cucumber female breeding, can solve the problems of backward breeding methods and low efficiency, and achieve the effect of shortening the breeding time

Inactive Publication Date: 2008-01-30
易克 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] One of the objectives of the present invention is to overcome the backwardness and low efficiency of existing cucumber female line breeding methods, and provide a female trait-related molecular marker for cucumber female line selection

Method used

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  • Cucumber female deseription related molecule label and uses thereof
  • Cucumber female deseription related molecule label and uses thereof
  • Cucumber female deseription related molecule label and uses thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1: Acquisition of Molecular Markers Related to Cucumber Female Traits

[0040] 1. Materials and Reagents

[0041] The pure female line WI1983G and the hermaphroditic flowering line WI1983H are a pair of near-isogenic materials, donated by Professor J.E. Staub, Department of Horticulture, U.S. Department of Agriculture, Agricultural Research Center, University of Wisconsin. The WI1983G female line is derived from the hybrid offspring system of WI3122 and WI3121 Advanced inbred lines. The hermaphrodite line WI1983H was derived from an inbred line that was resistant to wilting and hermaphrodite WI2189 as a donor and WI1983G after 5 generations of backcrossing and 3 generations of selfing. f 1 The generation is the first generation after crossing WI1983G and WI1983H. f 2 Substitute for F 1 Generation of self-bred offspring, a total of 156 strains.

[0042] 10×buffer, MgC l2 , dNTP, and TaqE were all purchased from Dalian Bao Biology Co., Ltd.; primers were sy...

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PUM

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Abstract

The invention discloses a molecular marker related to female traits of cucumber. The molecular marker is two SNP divergence sites of EIN3 gene of cucumber happening in places of 153bp and 501bp, wherein, the EIN3 gene of pure female plant has molecular markers of G153bp and T501bp or has any one of molecular markers of G153bp and T501bp. The method for obtaining the molecular marker is that first DNA group of young leaf of cucumber is extracted and the primer is designed to do PCR amplification, then a cloning sequence test for PCR products and comparison and analysis of a sequence test result are done and finally the primer is redesigned in parents and F2 groups to be verified. The molecular marker can be taken as specific marker for selection of female cucumber and applied to assisting selection of cucumber sex for breeding through gene marker, which significantly shortens breeding time.

Description

technical field [0001] The invention belongs to the technical field of molecular marker breeding, and in particular relates to a molecular marker related to cucumber female traits, a method for obtaining the molecular marker and an application of the marker in cucumber female breeding. Background technique [0002] Cucumber is one of the most cultivated vegetable crops in the world. Cucumber plants are rich in sex expression types. Female cucumbers refer to strains with only female flowers but not male flowers or a very small amount of male flowers. They often show the advantages of early maturity, dense melons, and high yield. Utilizing the female line to produce the first-generation hybrid does not require detasseling, which saves labor and reduces the cost of seed production, and the produced first-generation hybrid has high purity. The first-generation hybrids prepared with the female line as the female parent also show the advantages of early maturity, many female flow...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11A01H1/02
Inventor 徐向丽易克陈刚卢向阳
Owner 易克
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