Human papilloma virus sample particle vaccines

A human papillomavirus, virus-like technology, applied in the field of HPV vaccine, can solve the problem of inability to obtain HPV vaccine by preparation, and achieve the effect of improving preventive efficacy and preventing infection

Active Publication Date: 2008-02-06
CHANGCHUN BCHT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since it is currently impossible to obtain a large amount of HPV virus through tissue culture, and considering the potential carcinogenicity of HPVE6 and E7 genes, HPV vaccines cannot be obtained through traditional vaccine preparation methods such as attenuated live vaccines or inactivated vaccines

Method used

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  • Human papilloma virus sample particle vaccines
  • Human papilloma virus sample particle vaccines
  • Human papilloma virus sample particle vaccines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1HPV6L1VLPs, HPV16L1VLPs and HPV58L1VLPs mixed VLPs vaccine

[0021] The purified HPV6L1, HPV16L1 and HPV58L1 VLPs were formulated into a mixed vaccine at a ratio of 40 μg: 20 μg: 40 μg, and the adjuvant aluminum hydroxide was added at the same time, with a final concentration of about 1 mg / ml. After mixing thoroughly, they were stored at 4°C.

Embodiment 2

[0022] The preparation of embodiment 2 HPV6L1VLPs, HPV11L1VLPs, HPV16L1VLPs, HPV18L1VLPs and HPV58L1VLPs mixed VLPs vaccine

[0023] Prepare five VLPs of purified HPV6L1, HPV11L1, HPV16L1, HPV18L1 and HPV58L1 at the ratio of 40 μg: 40 μg: 20 μg: 40 μg: 40 μg to prepare a mixed vaccine, and add the adjuvant aluminum hydroxide at the same time, the final concentration is about 1 mg / ml, and mix well Afterwards, store at 4°C.

Embodiment 3

[0024] Example 3 Comparison of Immunogenicity of Monovalent VLPs and Mixed VLPs Vaccines

[0025] The prepared monovalent VLPs and mixed VLPs vaccines were inoculated into Balb / C mice respectively. The mice were divided into random groups, 5 mice in each group, and intraperitoneally inoculated on days 0, 14, and 28, respectively. Blood was collected 28 days after inoculation to detect antibody titer by ELISA method.

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Abstract

The invention relates to a virus-like particle vaccine against human papilloma virus, belonging to the biotechnology field. One milliliter vaccine contains the following components: HPV6L1VLPs: 10-60 ug, HPV16L1VLPs: 5-40 ug and HPV58L1VLPs10-60 ug. The invention has the advantages that HPV6L1, HPV16L1 and HPV58L1 proteins are secreted and expressed respectively, and the L1 proteins can be self-assembled to be VLPs. According to the infection epidemiology investigation result of human papilloma virus in some placed in Asia and China, the vaccine prepared by VLPs which is obtained by L1 proteins of HPV6, HPV16 and HPV58 can prevent 60 percent of the inventions resulting from HPV. The additional HPV type can further increases preventive effects of the vaccine.

Description

technical field [0001] The present invention relates to the preparation of HPV vaccine by using purified virus-like particles (VLPs) self-assembled into late protein L1 of different types of human papillomavirus (Human Papillomavirus, HPV) through proper ratio compatibility, and is used for preventing some diseases caused by HPV. Background technique [0002] HPV is a kind of double-stranded small molecule DNA virus with strict species specificity. It mainly infects human skin and mucous membrane tissues, causing proliferative lesions of epithelial tissues in corresponding parts. According to the site of infection, it can be divided into two groups: skin type and mucosal type. The mucosal type of HPV is divided into two types according to the nature of the lesions: the low-risk type that causes benign hyperplasia of the mucosal epithelium and the type that is associated with various human organ malignant tumors (such as cervical cancer) , penile cancer, laryngeal and head an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/23A61P35/00
Inventor 孔维刘大维
Owner CHANGCHUN BCHT BIOTECH
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