The invention provides a porcine circovirus 3 Cap protein, nucleic acid, virus-like particles, vaccine, and a preparation method and application of the porcine circovirus 3 Cap protein, and relates tothe technical field of molecular biology. The porcine circovirus 3 Cap protein provided by the invention has an amino acid sequence shown as SEQ ID NO.2, can be expressed in a soluble manner in a yeast expression system, and effectively improves yeast expression efficiency of the porcine circovirus 3 Cap protein. In addition, the porcine circovirus 3 Cap protein provided by the invention can be self-assembled into the virus-like particles, the vaccine prepared by using the virus-like particles has the characteristics of cell immunity and humoral immunity, and immunologic related experiments show that the immune effect is good. The method for expressing the porcine circovirus type 3 Cap protein by utilizing the yeast expression system has the advantages that production cost is low, the production process is simple, and a large amount of target proteins can be obtained through high-density fermentation, has no endotoxin pollution, and can be used for large-scale production.