Respiratory syncytial virus virus-like particle vaccine as well as preparation method and application thereof

Abstract

The invention belongs to the field of biotechnology, and particularly relates to a respiratory syncytial virus (RSV) virus-like particle (VLPs) subunit vaccine as well as a preparation method and application thereof. The component of the vaccine is chimeric antigen protein which is fusion-expressed together with neutral antigenic epitope of an RSVG protein or simultaneously with the antigenic epitope of T cells of an M2 protein by taking hepatitis B virus core protein as a carrier. The high-purity antigen component is prepared by efficiently expressing antigen protein in escherichia coli and then performing in-vitro purification, degeneration and renaturation and self assembling into virus-like particles (VLPs). The RSVG protein contained in the VLPs and the antigenic epitope of the T cells of the M2 protein are simultaneously expressed, so that the capability of the vaccine for introducing specific immunity response and anti-RSV infection immunity protection can be enhanced, the balanced Th1 / Th2 immunity response can be induced and the RSV vaccine is prevented from enhancing the incidence of diseases. Animals are immunized and inoculated with VLPs vaccines to induce organisms to generate high-level RSV neutral antigens, enhanced Th1 cell factor level and effective protection on RSV attack infection.

Description

technical field

[0001] The invention relates to a virus-like particle vaccine chimerically expressing multi-epitope antigens of respiratory syncytial virus and its preparation method and application, which belongs to the field of biotechnology and is used for the immune prevention of respiratory syncytial virus infection of humans and susceptible animals. Background technique

[0002] Respiratory syncytial virus (Respiratory Syncytial Virus, RSV) is one of the important pathogens that cause lower respiratory tract infection in infants and young children worldwide and cause them to suffer from bronchiolitis and pneumonia, which can seriously cause bronchial asthma and even death. Almost all children before the age of two Have had at least one RSV infection (Black, 2003). The younger the age, the more severe the disease, the higher the reinfection rate, which is closely related to the occurrence of asthma; the immunocompromised are more susceptible to infection, and the clinic...

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