Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of universal novel coronavirus vaccine and preparation method thereof

A general-purpose coronavirus technology, applied in the field of biomedicine, can solve problems such as single potency, high cost, and easy escape of viruses, so as to solve the risks of vaccine cell preparation and safety control, quickly realize large-scale industrial production, and solve Effects of Safety and High Cost Concerns

Active Publication Date: 2022-07-12
BEIJING MEIKANG JIMIAN BIOTECH CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest challenge for vaccine development is to find a suitable animal model. At present, non-human primate infection models are mainly used to verify the safety and efficacy of vaccine development, but the cost is extremely high and time-consuming
[0004] In the existing virus vaccine technology, the whole virus antigen vaccine uses the traditional inactivated virus or attenuated virus as the antigen, which has the problems of low safety and high cost; the virus specific antigen vaccine uses the synthetic S protein as the antigen, The artificially synthesized S protein is mainly expressed by bacteria, yeast, cells, or viral vectors, which has the problems of long time-consuming, single titer, easy virus escape, short validity period, and high cost; DNA and mRNA vaccines need to enter cells and be injected into the body. It has an immune effect only after it is converted into a protein. The problem of immunogenicity is the bottleneck that this technology cannot break through at present.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of universal novel coronavirus vaccine and preparation method thereof
  • A kind of universal novel coronavirus vaccine and preparation method thereof
  • A kind of universal novel coronavirus vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1 Design of fusion protein

[0085] Comparing the nucleic acid sequence of the new coronavirus SARS-CoV-2 with SARS and MERS viruses, the SARS-CoV-2 virus mainly expresses four structural proteins S protein (surface glycoprotein), E protein (Envelop protein), M protein (Membrane protein) protein), N protein (nucleocapsid phosphoprotein) and a protease ORF1a polyprotein (polyprotein cleavage protease);

[0086] In this example, according to the published full-length 1273aa amino acid sequence of the S protein (MN908947.3), the S protein signal peptide (SEQ ID NO: 2) and the S protein receptor binding domain (SEQ ID NO: 1) were selected as the first part of the fusion protein. One part, select E protein amino-terminal 29aa peptide segment (SEQ ID NO:4) as the second part of fusion protein, select M protein carboxyl-terminal 58aa peptide segment (SEQ ID NO:5) as the third part of fusion protein, select N protein The middle 162aa helix-turn domain (SEQ ID NO:7) is ...

Embodiment 2

[0089] Example 2 Design of mini gene

[0090] The amino acid sequence of fusion protein SEMNP is converted into corresponding nucleic acid sequence, and the fusion protein SEMNP coding gene shown in SEQ ID NO: 12 is obtained after optimization;

[0091] The 3'-end NP sequence (nt.4990-5840, NheI-SpeI deletion) of the SEMNP-encoding gene was deleted to obtain the fusion protein SEM-encoding gene shown in SEQ ID NO: 11;

[0092] The 3'-end EMNP sequence (nt.4680-5850, BstBI-BstBIdeletion) of the SEMNP encoding gene was deleted to obtain the fusion protein S encoding gene shown in SEQ ID NO: 10.

Embodiment 3

[0093] Example 3 Construction of lentiviral vector

[0094] Artificially synthesize the encoding genes of fusion proteins S, SEM and SEMNP shown in SEQ ID NO: 10, SEQ ID NO: 11 or SEQ ID NO: 12, double-enzymatic digestion of the above nucleic acid molecules, incubated in a 37°C water bath for 30min, DNA electrophoresis was performed on the digested product in a 1.5% agarose gel, and the digested fragment was purified and recovered using an agarose gel kit;

[0095] like figure 2 As shown, the restriction fragment was inserted into the linearized lentiviral vector TYF to construct the lentiviral vectors LV-S, LV-SEM and LV-SEMNP containing the mini gene.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a universal novel coronavirus vaccine and a preparation method thereof. The vaccine is an artificial fusion protein expressing novel coronavirus structural proteins S protein, E protein, M protein, N protein and non-structural protein ORF1a polyprotein. Antigen-presenting cells, the vaccine mimics the body's natural immune system. In the presence of cytokines, various polypeptide fragments formed by fusion proteins are presented by antigen-presenting cells, which can stimulate the body to produce immune responses and form immune responses. memory, and has broad-spectrum immune stimulation; the vaccine can realize rapid and large-scale industrial production, and has the advantages of high safety and low cost.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a universal novel coronavirus vaccine and a preparation method thereof. Background technique [0002] The key to the infection of human cells by the new coronavirus (SARS-CoV-2) lies in the binding of the S protein to the ACE2 protein. In SARS virus and MERS virus, S protein also plays the same role. Therefore, the S protein determines the host range and specificity of the virus, is an important action site for antibodies in the host, and is also a key target for vaccines, therapeutic antibodies, and diagnostic reagents. [0003] The virus that causes COVID-19 is a newly emerged variant of the coronavirus. The spike protein (S protein) of the virus has a stronger binding ability to the receptor ACE2 than SARS-CoV, which explains the strong infectivity of the new coronavirus. The novel coronavirus stimulates the innate immune system of infected people, causing the body to rele...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/165C07K19/00C12N15/62C12N15/867C12N7/01C12N5/10A61K39/215A61P31/14
CPCC07K14/005C12N15/86C12N7/00A61K39/12A61P31/14C12N2770/20022C12N2770/20034C12N2740/15021C12N2740/15043C07K2319/02A61K2039/5252A61K39/4622A61K39/461A61K39/4615A61K39/4634A61K39/464838A61K2039/55522C07K2319/40C12N2740/16043
Inventor 张隆基
Owner BEIJING MEIKANG JIMIAN BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com