Maize inbred line resistant to MRDV bred by using molecule making

A molecular marker and inbred line technology, which is applied in the field of maize breeding and plant bioengineering, can solve the problems of difficult disease resistance identification, time-consuming, labor-intensive and effective results of inbred lines and hybrids resistant to rough dwarf disease, and different positioning results.

Inactive Publication Date: 2008-03-12
SHANDONG UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0017] According to the report on the mapping of disease resistance genes, some problems can be found: 1) different mapping results may be obtained by using different hybrid combinations; 2) different positioning results may be obtained when identifying disease resistance in different environments; 3) different The type of QTL mapping population may affect the QTL mapping results; 4) The identification of disease resistance is a crucial factor affecting gene mapping
Maize rough dwarf disease is a complex trait that is difficult to identify for disease resistance. Conventional breeding methods, whether through natural disease identification in the field or through artificial inoculation to identify disease resistance, are important in breeding rough dwarf disease-resistant inbred lines and hybrids. It is time-consuming and labor-intensive with poor results
So far, there has been no report on the use of molecular marker-assisted selection to breed maize inbred lines resistant to rough dwarf disease

Method used

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  • Maize inbred line resistant to MRDV bred by using molecule making

Examples

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Effect test

example 1

[0068] Example 1: Breeding Ye 478 excellent improved line resistant to corn rough dwarf disease

[0069] Using Ye 478 or its improved line as the recipient and recurrent parent, and the inbred line 90110 as the donor of the disease resistance gene locus, excellent improved lines were obtained mainly through the prospect selection of the target gene.

[0070] Procedure one:

[0071] 1) Crossbreed with Ye 478 as the female parent and 90110 as the male parent to produce F 1 plants. This step can be completed in the greenhouse or in Hainan from January to April of the first year.

[0072] 2)F 1 Plant selfing produces F 2 seed. This step is completed in northern my country from May to August of the first year.

[0073] 3) F2 seeds were sown in flower pots, leaf DNA was extracted at the 3-leaf stage of the plants, PCR amplification was performed with primers of SSR markers umc1656, bnlg2191, umc1401, umc1666, bnlg1823 and umc1268 respectively, and the reaction products were su...

example 2

[0090] Example 2: Breeding an excellent improved line of Ye 502 resistant to corn rough dwarf disease

[0091] Using Ye 502 or its improved line as the recipient and recurrent parent, and the inbred line 90110 as the donor of the disease resistance gene locus, excellent improved lines were obtained mainly through the prospect selection of the target gene. Because Ye 502 has poor disease resistance, it is the best plan to improve multiple traits.

[0092] Procedure one:

[0093] 1) F was produced by crossing Ye 502 as the female parent and 90110 as the male parent 1 plants. This step can be completed in the greenhouse or in Hainan from January to April of the first year.

[0094] 2)F 1 Plant selfing produces F 2 seed. This step is completed in northern my country from May to August of the first year.

[0095] 3) F2 seeds were sown in flower pots, leaf DNA was extracted at the 3-leaf stage of the plants, PCR amplification was performed with primers of SSR markers umc1656,...

example 3

[0113] Example 3: Breeding excellent inbred lines resistant to maize rough dwarf disease

[0114] With excellent inbred lines DH4866 and Zheng 58 as recipients and recurrent parents, and inbred line 90110 as donor of disease resistance genes, excellent improved lines with different characteristics were obtained mainly through prospect selection of target genes. Proceed as follows:

[0115] 1) Crossbreed DH4866 and Zheng 58 as the father and mother respectively to produce F 1 seed. This step can be completed in the greenhouse or in Hainan from January to April of the first year.

[0116] 2)F 1 Plant selfing produces F 2 generation seed, from F 2 F 3 Substitute seeds are generally controlled at 30 to 40 ears. This step is completed in northern my country and / or Hainan from May to December of the first year.

[0117] 3)F 3 Plant selective selfing produces F 4 Substitute seeds, and harvest 40-60 ears. This step is completed in the greenhouse or in Hainan from January to A...

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Abstract

The present invention discloses a corn inbred method for selecting the anti-rough dwarf disease corns which utilizes the molecule marking at the rough dwarf disease resistance points of corns, i.e. the molecule marks on the three points from the inbred rough dwarf disease resistant corns are applied for rough dwarf disease resistant corns breeding and gene pyramiding breeding. The rough dwarf disease resistant and rough dwarf disease sensing corns are taken as the materials for building and separating the groups; the closely linked molecule marks of umc1656, bnlg2191, umc1401, umc1666, bnlg1823 and umc1268 of three rough dwarf disease resistant gene points are used for auxiliary choice; plants with three or two disease resistant gene points are taken as the chosen material for backcrossing and inbreeding; with the comprehensive choice and homozygosis, the high-quality inbred corns of the high property of rough dwarf disease resistance are obtained.

Description

technical field [0001] The invention belongs to the fields of corn breeding and plant bioengineering, and in particular relates to a method for breeding corn inbred lines resistant to rough dwarf disease by using molecular markers of corn rough dwarf disease resistance loci. Background technique [0002] Research progress of maize rough dwarf disease: [0003] 1. Hazards and causes of rough dwarf disease of corn [0004] Maize Rough Dwarf Disease (MRDD) is a viral disease that seriously endangers maize production. Symptoms of rough dwarf disease of corn are small transparent dotted dots between the fine veins on both sides of the midrib of the young leaves, and the transparent dots gradually increase, waxy white protrusions of different thicknesses appear on the veins on the back of the leaves, and there is obvious roughness when touched Infected plants are dwarfed, with shortened internodes; leaves are dark green; leaves are short, wide and thick, stiff and erect, often w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12N15/65
Inventor 张举仁杨爱芳张可炜
Owner SHANDONG UNIV
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